What the test is (and isn’t) Test design & intended use. The Canivet Asian4 VetDx (VD041) is a lateral-flow immunochromatographic combo assay designed to detect Ehrlichia canis antibodies (EHR Ab), Babesia gibsoni antibodies (BAB Ab), Anaplasma spp. antibodies (ANA Ab) and canine heartworm (Dirofilaria immitis) antigen (CHW Ag) in dog whole blood, serum, or plasma; […]
Author: Katelyn
Applications of High-Fidelity PCR in Next-Generation Sequencing Library Preparation
Introduction Next-generation sequencing (NGS) has revolutionized genomics by enabling massively parallel analysis of DNA and RNA with single-base resolution. Yet, the accuracy of downstream results is only as reliable as the upstream library preparation workflows. Among the most critical steps is PCR amplification, which enriches DNA fragments after adapter ligation and ensures sufficient yield for […]
Manufacturing Considerations for GMP Recombinant Human GM-CSF: From Expression System to Fill-Finish
Product & Platform Fundamentals GM-CSF biology & structure. Human GM-CSF is a 127-aa cytokine with two intramolecular disulfide bonds and potential N-/O-glycosylation. Glycoforms and disulfide pairing influence receptor affinity (GM-CSF-Rα/βc), potency, and clearance. Commercial exemplars illustrate platform impact: sargramostim is a yeast-derived, glycosylated recombinant GM-CSF (Leukine) with microheterogeneous glycoforms; molgramostim is E. coli-derived, non-glycosylated GM-CSF […]
Optimizing Experimental Conditions for Accurate Cell Proliferation Measurement with CCK-8
Introduction Accurate quantification of cell proliferation is a cornerstone of experimental biology, spanning fields from cancer pharmacology to stem cell biology. Among available tools, the Cell Counting Kit-8 (CCK-8) has become widely adopted because of its ease of use, sensitivity, and compatibility with high-throughput applications (NIH Assay Guidance Manual, NCI Developmental Therapeutics Program). The assay […]
Optimizing Stem Cell Culture with Matrix GFR, LDEV-Free: Best Practices and Protocols
Stem cell research continues to advance at a rapid pace, fueling breakthroughs in regenerative medicine, drug discovery, and disease modeling. One key area that significantly influences the success of stem cell cultures is the substrate used for cell attachment, growth, and differentiation. Matrix GFR, LDEV-Free, a recombinant form of extracellular matrix proteins, is widely utilized […]
Understanding the Chemistry: How AffiDYE Plus Achieves Tri-Color Labeling for Protein Markers
The AffiDYE Plus system is a revolutionary advancement in protein labeling technology, enabling tri-color labeling of proteins for more efficient and precise analysis in various biological research applications. Tri-color labeling is a powerful tool, especially for multiplex assays, as it allows researchers to detect and differentiate multiple protein targets in a single sample. In this […]
Streamlining Proteomics Sample Prep: Integrating Precast Protein Plus Gels in LC-MS-Compatible Workflows
Proteomics has revolutionized the way scientists understand complex biological systems by allowing the detailed analysis of proteins, their functions, interactions, and structures. The combination of Liquid Chromatography-Mass Spectrometry (LC-MS) has become one of the most powerful techniques in proteomics research. However, one of the most critical and often limiting steps in any LC-MS workflow is […]