Vibrio cholerae infects human hosts following ingestion of contaminated meals or water, ensuing within the extreme diarrheal illness cholera. The watery diarrhea that’s attribute of the illness is immediately brought on by manufacturing of cholera toxin (CT). A fancy regulatory cascade controls manufacturing of CT and different virulence elements. Nevertheless, finally a single protein, ToxT, immediately binds to virulence gene promoters and prompts their transcription. Beforehand, we recognized two ToxT binding websites, or toxboxes, inside the cholera toxin promoter (PctxAB). The toxboxes overlap with the 2 promoter-proximal GATTTTT heptad repeats discovered inside PctxAB in classical biotype V. cholerae pressure O395.
These heptad repeats had been beforehand discovered to be inside a big DNA area sure by H-NS, a worldwide transcriptional repressor current in Gram-negative micro organism. The present mannequin for management of PctxAB transcription proposed full H-NS displacement from the DNA by ToxT, adopted by direct activation by ToxT-RNAP contacts. The aim of this examine was to find out extra exactly the place H-NS binds to PctxAB and check the speculation that ToxT fully displaces H-NS from the PctxAB promoter earlier than activating transcription.
Outcomes recommend that H-NS binds solely to the area of PctxAB encompassing the heptad repeats and ToxT solely displaces H-NS from its most promoter proximal binding websites, calling for a revision of the present mannequin involving H-NS and ToxT at PctxAB. H-NS is a worldwide detrimental regulator of transcription in Gram detrimental micro organism, significantly in horizontally acquired genetic islands. Earlier work in Vibrio cholerae recommended that H-NS represses transcription of cholera toxin genes by binding to a big area upstream of its promoter, and that virulence activator ToxT derepresses transcription by eradicating H-NS from the promoter.
Right here, new knowledge assist a revised mannequin wherein ToxT solely displaces H-NS sure to essentially the most promoter proximal DNA websites that overlap the ToxT binding websites, leaving the upstream websites occupied by H-NS. This introduces the next decision mechanism for antirepression of H-NS in charge of cholera toxin manufacturing.
Growth of DNA Markers From Bodily Mapped Loci in Aegilops comosa and Aegilops umbellulata Utilizing Single-Gene FISH and Chromosome Sequences
Breeding of agricultural crops tailored to local weather change and proof against ailments and pests is hindered by a restricted gene pool due to domestication and hundreds of years of human choice. One approach to enhance genetic variation is chromosome-mediated gene switch from wild family by cross hybridization. Within the case of wheat (Triticum aestivum), the species of genus Aegilops are a very enticing supply of latest genes and alleles. Nevertheless, through the evolution of the Aegilops and Triticum genera, diversification of the D-genome lineage resulted within the formation of diploid C, M, and U genomes of Aegilops.
The extent of structural genome alterations, which accompanied their evolution and speciation, and the scarcity of molecular instruments to detect Aegilops chromatin hamper gene switch into wheat. To analyze the chromosome construction and assist develop molecular markers with a identified bodily place that might enhance the effectivity of the choice of desired introgressions, we developed single-gene fluorescence in situ hybridization (FISH) maps for M- and U-genome progenitors, Aegilops comosa and Aegilops umbellulata, respectively. Forty-three ortholog genes had been situated on 47 loci in Ae. comosa and on 52 loci in Ae. umbellulata utilizing wheat cDNA probes.
The outcomes obtained confirmed that M-genome chromosomes preserved collinearity with these of wheat, excluding 2 and 6M containing an intrachromosomal rearrangement and paracentric inversion of 6ML, respectively. Whereas Ae. umbellulata chromosomes 1, 3, and 5U maintained collinearity with wheat, structural reorganizations in 2, 4, 6, and 7U recommended a similarity with the C genome of Aegilops markgrafii. To develop molecular markers with precise bodily positions on chromosomes of Aegilops, the single-gene FISH knowledge had been validated in silico utilizing DNA sequence assemblies from flow-sorted M- and U-genome chromosomes.
The sequence similarity search of cDNA sequences confirmed 44 out of the 47 single-gene loci in Ae. comosa and 40 of the 52 map positions in Ae. umbellulata. Polymorphic areas, thus, recognized enabled the event of molecular markers, which had been PCR validated utilizing wheat-Aegilops disomic chromosome addition traces. The one-gene FISH-based strategy allowed the event of PCR markers particular for cytogenetically mapped positions on Aegilops chromosomes, substituting as but unavailable segregating map. The brand new information and assets will assist the efforts for the introgression of Aegilops genes into wheat and their cloning.
Terminal Sequence-Particular Interparticle Attraction between DNA Duplex-Carrying Polystyrene Microparticles in Aqueous Salt Answer Assessed by Optical Tweezers
The dispersion conduct of DNA duplex-carrying colloidal particles in aqueous high-salt options exhibits extraordinary selectivity in opposition to the duplex terminal sequence. We investigated the interparticle drive between DNA duplex-carrying polystyrene (dsDNA-PS) microparticles in aqueous salt options and examined their conduct in relation to the duplex terminal sequences. Drive-distance (F-D) curves for a pair of dsDNA-PS particles had been recorded with a dual-beam optical tweezers system with the 2 optically trapped particles intently approaching one another.
The egg drop syndrome (76) PCR kit |
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PCR-V044-48D | Bioingentech | 50T | EUR 543.6 |
The egg drop syndrome (76) PCR kit |
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PCR-V044-96D | Bioingentech | 100T | EUR 686.4 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-100D | Bioingentech | 100T | EUR 1039.2 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-150D | Bioingentech | 150T | EUR 1177.2 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-50D | Bioingentech | 50T | EUR 861.6 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-100D | Bioingentech | 100T | EUR 860.4 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-150D | Bioingentech | 150T | EUR 969.6 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-50D | Bioingentech | 50T | EUR 717.6 |
Probe PCR Mix |
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abx461071-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe PCR Mix |
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abx461071-596tests | Abbexa | 5 × 96 tests | Ask for price |
Probe PCR Mix |
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abx461071-96tests | Abbexa | 96 tests | EUR 1437.5 |
Ready? PCR Mix |
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M1127-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix |
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M1127-200 | Biovision | each | EUR 229.2 |
Rigor? PCR Mix |
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M1132-200 | Biovision | each | EUR 385.2 |
Breeze? PCR Mix |
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M1134-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix |
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M1136-200 | Biovision | each | EUR 352.8 |
Advance? PCR Mix |
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M1139-200 | Biovision | each | EUR 451.2 |
The egg drop syndrome (76) One-Step PCR kit |
|||
Oneq-V044-100D | Bioingentech | 100T | EUR 1039.2 |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-150D | Bioingentech | 150T | EUR 1177.2 |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-50D | Bioingentech | 50T | EUR 861.6 |
PCR Super Mix |
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abx299726-100g | Abbexa | 100 µg | Ask for price |
PCR Super Mix |
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abx299726-20g | Abbexa | 20 µg | EUR 362.5 |
PCR Super Mix |
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abx299726-50g | Abbexa | 50 µg | Ask for price |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Fire Start? PCR Mix |
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M1141-200 | Biovision | each | EUR 451.2 |
Ready? PCR Mix-Dye |
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M1128-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix-Dye |
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M1128-200 | Biovision | each | EUR 229.2 |
Image Ready? PCR Mix |
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M1129-200 | Biovision | each | EUR 352.8 |
Rigor? PCR Mix-Dye |
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M1133-200 | Biovision | each | EUR 385.2 |
Whole Blood PCR Mix |
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M1143-200 | Biovision | each | EUR 385.2 |
PowerPol 2X PCR Mix |
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RK20718 | Abclonal | 1mL | EUR 23.44 |
Robust Ready? PCR Mix |
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M1130-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix |
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M1130-200 | Biovision | each | EUR 255.6 |
Breeze? PCR Mix-Dye |
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M1135-200 | Biovision | each | EUR 320.4 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-1 | Sisco Laboratories | 5 x1 ml | EUR 64.14 |
Description: Part E |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-2 | Sisco Laboratories | 5 x2.5 ml | EUR 128.29 |
Description: Part E |
Distant? PCR Mix-Dye |
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M1137-200 | Biovision | each | EUR 352.8 |
Image Distant? PCR Mix |
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M1138-200 | Biovision | each | EUR 385.2 |
Advance? PCR Mix-Dye |
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M1140-200 | Biovision | each | EUR 451.2 |
2x PCR Red Mix |
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TBS4004 | Tribioscience | 2.0 ml | EUR 88 |
2x PCR Blue Mix |
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TBS4005 | Tribioscience | 2.0 ml | EUR 88 |
PCR-EZ D-PCR MASTER MIX |
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BS294 | Bio Basic | 100RXN, 100prep | EUR 112.2 |
2X PCR Master Mix |
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28007 | Norgen Biotek Corp | 100 Reactions | EUR 47.4 |
2X PCR Master Mix |
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28009 | Norgen Biotek Corp | 500 Reactions | EUR 209.4 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
HRM PCR Master Mix |
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E0440-01 | EURx | 100reactions | EUR 55.59 |
Description: For detection of gene mutations and SNPs by HRM analysis |
HRM PCR Master Mix |
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E0440-02 | EURx | 500reactions | EUR 251.79 |
Description: For detection of gene mutations and SNPs by HRM analysis |
MGI PCR Primer Mix |
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RK20272 | Abclonal | 40μL | EUR 6 |
PCR Master mix (SYBR) |
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abx460031-1096tests | Abbexa | 10 × 96 tests | Ask for price |
PCR Master mix (SYBR) |
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abx460031-596tests | Abbexa | 5 × 96 tests | Ask for price |
PCR Master mix (SYBR) |
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abx460031-96tests | Abbexa | 96 tests | EUR 337.5 |
amfiSure PCR Master Mix |
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P0311-010 | GenDepot | 2x50 rxns | EUR 66 |
amfiSure PCR Master Mix |
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P0311-025 | GenDepot | 5X50 rxns | EUR 99 |
amfiSure PCR Master Mix |
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P0311-050 | GenDepot | 10X50 rxns | EUR 149 |
amfiSure PCR Master Mix |
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P0311-100 | GenDepot | 10x1ml | EUR 220 |
amfiSure PCR Master Mix |
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P0311-125 | GenDepot | 15X50 rxns | EUR 198 |
amfiSure PCR Master Mix |
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P0311-200 | GenDepot | 20x1ml | EUR 365 |
amfiSure PCR Master Mix |
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P0311-250 | GenDepot | 50x50 rxns | EUR 595 |
amfiSure PCR Master Mix |
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P0311-500 | GenDepot | 100x50 rxns | EUR 1010 |
amfiXpand PCR Master Mix |
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P0331-010 | GenDepot | 2X50 rxns | EUR 65 |
amfiXpand PCR Master Mix |
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P0331-025 | GenDepot | 5X50 rxns | EUR 118 |
amfiXpand PCR Master Mix |
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P0331-050 | GenDepot | 10X50 rxns | EUR 149 |
amfiXpand PCR Master Mix |
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P0331-250 | GenDepot | 50x50 rxns | EUR 430 |
PCR-Mix |
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L5051100 | Biochain | 2.5 ml | EUR 52.5 |
Taq 2X PCR Mix V2 |
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RK20607 | Abclonal | 5mL | EUR 3.82 |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503 | Sisco Laboratories | 1 ml | EUR 23.09 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503-1 | Sisco Laboratories | 5 x1 ml | EUR 70.13 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
|||
46503-2 | Sisco Laboratories | 5 x2.5 ml | EUR 145.39 |
Description: Part E |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
|||
MBS652948-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
|||
MBS652948-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
|||
MBS652948-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
|||
MBS652948-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
MyTaq Blood PCR Mix, 2x |
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BIO-25053/S | Bioline | Sample | Ask for price |
MyTaq Blood PCR Mix, 2x |
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BIO-25054 | Bioline | 250 Reactions | Ask for price |
Taq 2x PCR Mix 100 rxn |
|||
BA01501 | Bioatlas | 100rxn | EUR 112.8 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653231-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653231-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653231-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653231-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653231-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653424-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653424-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653424-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653424-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653424-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Fire Start? PCR Mix-Dye |
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M1142-200 | Biovision | each | EUR 451.2 |
HiScript-TS 2 × PCR Mix |
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RA103-01 | Vazyme | 40 rxns (25 μl/rxn) | EUR 87 |
HiScript-TS 2 × PCR Mix |
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RA103-02 | Vazyme | 200 rxns (25 μl/rxn) | EUR 344 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
HotTaq 2x PCR Mix 100 rxn |
|||
BA01503 | Bioatlas | 100rxn | EUR 144 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
RedTaq 2x PCR Mix 100 rxn |
|||
BA01507 | Bioatlas | 100rxn | EUR 175.2 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Robust Ready? PCR Mix-Dye |
|||
M1131-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix-Dye |
|||
M1131-200 | Biovision | each | EUR 255.6 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
mRNAExpress Tail PCR mix (5uM) |
|||
MR-TAIL-PR | SBI | 100 ul | EUR 111 |
2x Taq PCR Master Mix |
|||
A4145-1ML40Rxns | Biomatik Corporation | 1ML (40Rxns) | EUR 77 |
Description: Biotechnology |
Taq PCR Master Mix (2x) |
|||
E2520-01 | EURx | 100reactions | EUR 38.15 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq PCR Master Mix (2x) |
|||
E2520-02 | EURx | 200reactions | EUR 71.94 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq PCR Master Mix (2x) |
|||
E2520-03 | EURx | 500reactions | EUR 155.87 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq 2X PCR Master Mix |
|||
RK20602 | Abclonal | 500RXN | EUR 12.54 |
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder) |
|||
MBS652825-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder) |
|||
MBS652825-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder) |
|||
MBS652825-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder) |
|||
MBS652825-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder) |
|||
MBS652825-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Complete, Adenine Rich (2g), w/o Yeast Nitrogen Base (Powder) |
|||
MBS653317-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Complete, Adenine Rich (2g), w/o Yeast Nitrogen Base (Powder) |
|||
MBS653317-1kg | MyBiosource | 1kg | EUR 1180 |
Apparently, solely 3-5% of the oligo-DNA strands on the dsDNA-PS particles fashioned a duplex with complementary DNAs, and the F-D curves confirmed a definite specificity to the duplex terminal sequences within the interparticle drive at a high-NaCl focus; a transparent attraction peak was noticed in F-D curves solely when the duplex terminal was a complementary base pair.