Vibrio cholerae infects human hosts following ingestion of contaminated meals or water, ensuing within the extreme diarrheal illness cholera. The watery diarrhea that’s attribute of the illness is immediately brought on by manufacturing of cholera toxin (CT). A fancy regulatory cascade controls manufacturing of CT and different virulence elements. Nevertheless, finally a single protein, ToxT, immediately binds to virulence gene promoters and prompts their transcription. Beforehand, we recognized two ToxT binding websites, or toxboxes, inside the cholera toxin promoter (PctxAB). The toxboxes overlap with the 2 promoter-proximal GATTTTT heptad repeats discovered inside PctxAB in classical biotype V. cholerae pressure O395.
These heptad repeats had been beforehand discovered to be inside a big DNA area sure by H-NS, a worldwide transcriptional repressor current in Gram-negative micro organism. The present mannequin for management of PctxAB transcription proposed full H-NS displacement from the DNA by ToxT, adopted by direct activation by ToxT-RNAP contacts. The aim of this examine was to find out extra exactly the place H-NS binds to PctxAB and check the speculation that ToxT fully displaces H-NS from the PctxAB promoter earlier than activating transcription.
Outcomes recommend that H-NS binds solely to the area of PctxAB encompassing the heptad repeats and ToxT solely displaces H-NS from its most promoter proximal binding websites, calling for a revision of the present mannequin involving H-NS and ToxT at PctxAB. H-NS is a worldwide detrimental regulator of transcription in Gram detrimental micro organism, significantly in horizontally acquired genetic islands. Earlier work in Vibrio cholerae recommended that H-NS represses transcription of cholera toxin genes by binding to a big area upstream of its promoter, and that virulence activator ToxT derepresses transcription by eradicating H-NS from the promoter.
Right here, new knowledge assist a revised mannequin wherein ToxT solely displaces H-NS sure to essentially the most promoter proximal DNA websites that overlap the ToxT binding websites, leaving the upstream websites occupied by H-NS. This introduces the next decision mechanism for antirepression of H-NS in charge of cholera toxin manufacturing.
Growth of DNA Markers From Bodily Mapped Loci in Aegilops comosa and Aegilops umbellulata Utilizing Single-Gene FISH and Chromosome Sequences
Breeding of agricultural crops tailored to local weather change and proof against ailments and pests is hindered by a restricted gene pool due to domestication and hundreds of years of human choice. One approach to enhance genetic variation is chromosome-mediated gene switch from wild family by cross hybridization. Within the case of wheat (Triticum aestivum), the species of genus Aegilops are a very enticing supply of latest genes and alleles. Nevertheless, through the evolution of the Aegilops and Triticum genera, diversification of the D-genome lineage resulted within the formation of diploid C, M, and U genomes of Aegilops.
The extent of structural genome alterations, which accompanied their evolution and speciation, and the scarcity of molecular instruments to detect Aegilops chromatin hamper gene switch into wheat. To analyze the chromosome construction and assist develop molecular markers with a identified bodily place that might enhance the effectivity of the choice of desired introgressions, we developed single-gene fluorescence in situ hybridization (FISH) maps for M- and U-genome progenitors, Aegilops comosa and Aegilops umbellulata, respectively. Forty-three ortholog genes had been situated on 47 loci in Ae. comosa and on 52 loci in Ae. umbellulata utilizing wheat cDNA probes.
The outcomes obtained confirmed that M-genome chromosomes preserved collinearity with these of wheat, excluding 2 and 6M containing an intrachromosomal rearrangement and paracentric inversion of 6ML, respectively. Whereas Ae. umbellulata chromosomes 1, 3, and 5U maintained collinearity with wheat, structural reorganizations in 2, 4, 6, and 7U recommended a similarity with the C genome of Aegilops markgrafii. To develop molecular markers with precise bodily positions on chromosomes of Aegilops, the single-gene FISH knowledge had been validated in silico utilizing DNA sequence assemblies from flow-sorted M- and U-genome chromosomes.
The sequence similarity search of cDNA sequences confirmed 44 out of the 47 single-gene loci in Ae. comosa and 40 of the 52 map positions in Ae. umbellulata. Polymorphic areas, thus, recognized enabled the event of molecular markers, which had been PCR validated utilizing wheat-Aegilops disomic chromosome addition traces. The one-gene FISH-based strategy allowed the event of PCR markers particular for cytogenetically mapped positions on Aegilops chromosomes, substituting as but unavailable segregating map. The brand new information and assets will assist the efforts for the introgression of Aegilops genes into wheat and their cloning.
Terminal Sequence-Particular Interparticle Attraction between DNA Duplex-Carrying Polystyrene Microparticles in Aqueous Salt Answer Assessed by Optical Tweezers
The dispersion conduct of DNA duplex-carrying colloidal particles in aqueous high-salt options exhibits extraordinary selectivity in opposition to the duplex terminal sequence. We investigated the interparticle drive between DNA duplex-carrying polystyrene (dsDNA-PS) microparticles in aqueous salt options and examined their conduct in relation to the duplex terminal sequences. Drive-distance (F-D) curves for a pair of dsDNA-PS particles had been recorded with a dual-beam optical tweezers system with the 2 optically trapped particles intently approaching one another.
The egg drop syndrome (76) PCR kit |
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| PCR-V044-96D | Bioingentech | 100T | EUR 686.4 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-100D | Bioingentech | 100T | EUR 1039.2 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-150D | Bioingentech | 150T | EUR 1177.2 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-50D | Bioingentech | 50T | EUR 861.6 |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-100D | Bioingentech | 100T | EUR 860.4 |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-150D | Bioingentech | 150T | EUR 969.6 |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-50D | Bioingentech | 50T | EUR 717.6 |
The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-100D | Bioingentech | 100T | EUR 1039.2 |
The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-150D | Bioingentech | 150T | EUR 1177.2 |
The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-50D | Bioingentech | 50T | EUR 861.6 |
PCR Mix |
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| L5051100 | Biochain | 2.5 ml | EUR 67 |
PRICE DROP |
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| 110-012 | GeneOn | 4 x 1000 µl | EUR 159.6 |
PRICE DROP |
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| 110-012L | GeneOn | 5x 4 x 1000 µl | EUR 621.6 |
PRICE DROP |
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| 110-012XL | GeneOn | 10x 4 x 1000 µl | EUR 1126.8 |
Ready? PCR Mix |
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| M1127-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix |
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| M1127-200 | Biovision | each | EUR 229.2 |
Rigor? PCR Mix |
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| M1132-200 | Biovision | each | EUR 385.2 |
Breeze? PCR Mix |
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| M1134-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix |
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| M1136-200 | Biovision | each | EUR 352.8 |
Advance? PCR Mix |
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| M1139-200 | Biovision | each | EUR 451.2 |
Egg Drop Syndrome |
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| PCR-V026-PCRV02648D | Bioingentech | PCR-V026-48D | EUR 230 |
Egg Drop Syndrome |
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| PCR-V026-PCRV02696D | Bioingentech | PCR-V026-96D | EUR 312 |
Egg Drop Syndrome |
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| Oneq-V026-OneqV026100D | Bioingentech | Oneq-V026-100D | EUR 515 |
Egg Drop Syndrome |
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| Oneq-V026-OneqV026150D | Bioingentech | Oneq-V026-150D | EUR 594 |
Egg Drop Syndrome |
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| Oneq-V026-OneqV02650D | Bioingentech | Oneq-V026-50D | EUR 413 |
Ready PCR Mix 2X |
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| 11170052-1 | Bio-WORLD | 1 Unit (s) | EUR 46.41 |
Ready PCR Mix 2X |
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| 11170052-2 | Bio-WORLD | 2 Unit (s) | EUR 84.38 |
Fire Start? PCR Mix |
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| M1141-200 | Biovision | each | EUR 451.2 |
Ready? PCR Mix-Dye |
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| M1128-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix-Dye |
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| M1128-200 | Biovision | each | EUR 229.2 |
Image Ready? PCR Mix |
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| M1129-200 | Biovision | each | EUR 352.8 |
Rigor? PCR Mix-Dye |
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| M1133-200 | Biovision | each | EUR 385.2 |
Whole Blood PCR Mix |
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| M1143-200 | Biovision | each | EUR 385.2 |
Robust Ready? PCR Mix |
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| M1130-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix |
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| M1130-200 | Biovision | each | EUR 255.6 |
Breeze? PCR Mix-Dye |
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| M1135-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix-Dye |
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| M1137-200 | Biovision | each | EUR 352.8 |
Image Distant? PCR Mix |
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| M1138-200 | Biovision | each | EUR 385.2 |
Advance? PCR Mix-Dye |
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| M1140-200 | Biovision | each | EUR 451.2 |
PCR Master Mix 2X |
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| 11140011-1 | Bio-WORLD | 100 Reaction (s) | EUR 171.48 |
PCR Master Mix 2X |
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| 11140011-2 | Bio-WORLD | 200 Reaction (s) | EUR 323.39 |
PCR Master Mix 2X |
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| 11140011-3 | Bio-WORLD | 500 Reaction (s) | EUR 692.46 |
PCR-EZ D-PCR MASTER MIX |
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| BS294 | Bio Basic | 100RXN, 100prep | EUR 112.2 |
amfiSure PCR Master Mix |
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| P0311-010 | GenDepot | 2x50 rxns | EUR 151.2 |
amfiSure PCR Master Mix |
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| P0311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure PCR Master Mix |
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| P0311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure PCR Master Mix |
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| P0311-100 | GenDepot | 10x1ml | EUR 343.2 |
amfiSure PCR Master Mix |
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| P0311-125 | GenDepot | 15X50 rxns | EUR 333.6 |
amfiSure PCR Master Mix |
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| P0311-200 | GenDepot | 20x1ml | EUR 562.8 |
amfiSure PCR Master Mix |
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| P0311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure PCR Master Mix |
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| P0311-500 | GenDepot | 100x50 rxns | EUR 1509.6 |
Taq 2X PCR Mix V2 |
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| RK20607 | Abclonal | 5mL | EUR 4 |
amfiXpand PCR Master Mix |
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| P0331-010 | GenDepot | 2X50 rxns | EUR 181.2 |
amfiXpand PCR Master Mix |
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| P0331-025 | GenDepot | 5X50 rxns | EUR 327.6 |
amfiXpand PCR Master Mix |
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| P0331-050 | GenDepot | 10X50 rxns | EUR 537.6 |
amfiXpand PCR Master Mix |
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| P0331-250 | GenDepot | 50x50 rxns | EUR 2247.6 |
Taq 2x PCR Mix 100 rxn |
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| BA01501 | Bioatlas | 100rxn | EUR 112.8 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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MyTaq Blood PCR Mix, 2x |
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| BIO-25053/S | Bioline | Sample | Ask for price |
MyTaq Blood PCR Mix, 2x |
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| BIO-25054 | Bioline | 250 Reactions | Ask for price |
HotTaq 2x PCR Mix 100 rxn |
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| BA01503 | Bioatlas | 100rxn | EUR 144 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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RedTaq 2x PCR Mix 100 rxn |
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| BA01507 | Bioatlas | 100rxn | EUR 175.2 |
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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Fire Start? PCR Mix-Dye |
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| M1142-200 | Biovision | each | EUR 451.2 |
HiScript-TS 2 × PCR Mix |
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| RA103-01 | Vazyme | 40 rxns (25 μl/rxn) | EUR 69.6 |
HiScript-TS 2 × PCR Mix |
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| RA103-02 | Vazyme | 200 rxns (25 μl/rxn) | EUR 275.2 |
Robust Ready? PCR Mix-Dye |
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| M1131-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix-Dye |
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| M1131-200 | Biovision | each | EUR 255.6 |
Funnel Pyrex Cyl Drop Gr.500ml |
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| FUN4024 | Scientific Laboratory Supplies | EACH | EUR 176.4 |
mRNAExpress Tail PCR mix (5uM) |
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| MR-TAIL-PR | SBI | 100 ul | EUR 111 |
2x Taq PCR Master Mix |
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| A4145-1ML40Rxns | Biomatik Corporation | 1ML (40Rxns) | EUR 77 |
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Description: Biotechnology |
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Drop Delay Calibration Particles |
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| DDCP-70-2 | Spherotech | 2 mL | EUR 339.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Drop Delay Calibration Particles |
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| DDCP-70-20 | Spherotech | 20 mL | EUR 2301.6 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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The egg drop syndrome (76) |
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| PCR-V044-PCRV04448D | Bioingentech | PCR-V044-48D | EUR 230 |
The egg drop syndrome (76) |
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| PCR-V044-PCRV04496D | Bioingentech | PCR-V044-96D | EUR 312 |
The egg drop syndrome (76) |
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| Oneq-V044-OneqV044100D | Bioingentech | Oneq-V044-100D | EUR 515 |
The egg drop syndrome (76) |
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| Oneq-V044-OneqV044150D | Bioingentech | Oneq-V044-150D | EUR 594 |
The egg drop syndrome (76) |
|||
| Oneq-V044-OneqV04450D | Bioingentech | Oneq-V044-50D | EUR 413 |
Taq 5x PCR Mix 12.5 mM |
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| BT10402 | Bioatlas | 250rxn | EUR 130.8 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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EntiLink™ PCR Master Mix |
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| EQ004 | ELK Biotech | 1mL | EUR 16 |
Drop Funnel Rotaflo SC 50ml |
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| QD312 | Scientific Laboratory Supplies | PK5 | EUR 637.2 |
amfiSure Prime PCR Master Mix |
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| P1311-025 | GenDepot | 5X50 rxns | EUR 193.2 |
amfiSure Prime PCR Master Mix |
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| P1311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure Prime PCR Master Mix |
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| P1311-125 | GenDepot | 15X50 rxns | EUR 327.6 |
amfiSure Prime PCR Master Mix |
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| P1311-250 | GenDepot | 50x50 rxns | EUR 886.8 |
amfiSure Prime PCR Master Mix |
|||
| P1311-500 | GenDepot | 100x50 rxns | EUR 1485.6 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-050 | GenDepot | 10X50 rxns | EUR 267.6 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-125 | GenDepot | 15X50 rxns | EUR 338.4 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure Advanced PCR Master Mix |
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| P2311-500 | GenDepot | 100x50 rxns | EUR 1534.8 |
TruePol 2X PCR Mix for Microbiome |
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| RK20720 | Abclonal | 200RXN | EUR 107.5 |
Funnel Pyrex Cyl Drop Gr 1Ltr |
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| FUN4026 | Scientific Laboratory Supplies | EACH | EUR 249.6 |
Taq 2X PCR Mix with Dye V2 |
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| RK20608 | Abclonal | 5mL | EUR 151.5 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-005 | GenDepot | 5x1 ml | EUR 262.8 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-010 | GenDepot | 10x1 ml | EUR 380.4 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-050 | GenDepot | 50x1 ml | EUR 1453.2 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-100 | GenDepot | 100x1 ml | EUR 2522.4 |
EntiLink™ PCR Master Mix(Red) |
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| EQ027 | ELK Biotech | 1mL | EUR 16 |
Drop Funnel Cyl Rota S/C 100ml |
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| QD1/21GP | Scientific Laboratory Supplies | EACH | EUR 109.2 |
Drop Funnel Cyl Rota S/C 100ml |
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| QD1/22GP | Scientific Laboratory Supplies | EACH | EUR 116.4 |
Drop Funnel Cyl Rota S/C 250ml |
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| QD1/32GP | Scientific Laboratory Supplies | EACH | EUR 166.8 |
Drop Funnel Cylind Rota S/C 50ml |
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| QD1/11GP | Scientific Laboratory Supplies | EACH | EUR 102 |
Taq PCR Master Mix (2X, Red Dye) |
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| BS9297 | Bio Basic | 1ml | EUR 101.76 |
Taq PCR Master Mix (2X, Red Dye) |
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| BS9298 | Bio Basic | 5ml | EUR 227.04 |
dNTP Mix（PCR Grade）10 mM each |
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| EQ011 | ELK Biotech | 100μl | EUR 12 |
Taq PCR Master Mix (2X, Blue Dye) |
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| BS9295 | Bio Basic | 1ml | EUR 101.76 |
Taq PCR Master Mix (2X, Blue Dye) |
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| BS9296 | Bio Basic | 5ml | EUR 227.04 |
2×Taq PCR Master Mix(with dye) |
|||
| K1034-1 | ApexBio | 1 ml | EUR 87.6 |
2×Taq PCR Master Mix(with dye) |
|||
| K1034-100 | ApexBio | 100×1 ml | EUR 950.4 |
2×Taq PCR Master Mix(with dye) |
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| K1034-20 | ApexBio | 20×1 ml | EUR 338.4 |
2×Taq PCR Master Mix(with dye) |
|||
| K1034-5 | ApexBio | 5×1 ml | EUR 142.8 |
2×Taq PCR Master Mix(with dye) |
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| K1034-50 | ApexBio | 50×1 ml | EUR 616.8 |
Taylor 5863 Splash and Drop Timer, each |
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| IW-5863 | IHC World | - | EUR 24.5 |
Watershed Hanging Drop Cover Slip Endstation |
|||
| M-WS180320-24WCSES | MiTeGen | 1 UNIT | EUR 256 |
|
Description: Watershed Hanging Drop Cover Slip Endstation |
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Gloria Nova HS 2X PCR Mix for NGS |
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| RK20716 | Abclonal | 24RXN | EUR 57 |
Drop Funnel P/Equal R S/C 100ml |
|||
| QDE100/22 | Scientific Laboratory Supplies | EACH | EUR 192 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | EUR 251.4 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | EUR 115.68 |
Drop-n-Stain EverBrite™ Mounting Medium |
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| 23008 | Biotium | 1ML | EUR 154 |
|
Description: N/A |
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Drop-n-Stain EverBrite™ Mounting Medium |
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| 23008-T | Biotium | 1ML | EUR 39 |
|
Description: N/A |
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amfiSure Ultra Fidelity PCR Master Mix(2X) |
|||
| P0346-001 | GenDepot | 1ml | EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|||
| P0346-002 | GenDepot | 2x1ml | EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|||
| P0346-005 | GenDepot | 5x1ml | EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|||
| P0346-010 | GenDepot | 10x1ml | EUR 1632 |
amfiFusion High Fidelity PCR Master Mix(2X) |
|||
| P0342-010 | GenDepot | 2x50 rxns | EUR 303.6 |
amfiFusion High Fidelity PCR Master Mix(2X) |
|||
| P0342-025 | GenDepot | 5X50 rxns | EUR 546 |
amfiFusion High Fidelity PCR Master Mix(2X) |
|||
| P0342-050 | GenDepot | 10X50 rxns | EUR 934.8 |
amfiFusion High Fidelity PCR Master Mix(2X) |
|||
| P0342-100 | GenDepot | 20x50 rxns | EUR 1599.6 |
Reverse Transcriptase RT PCR Master Mix (5X) |
|||
| MB1002-100reactions | BosterBio | 100 reactions | EUR 616.8 |
Reverse Transcriptase RT PCR Master Mix (5X) |
|||
| MB1002-200reactions | BosterBio | 200 reactions | EUR 895.2 |
Reverse Transcriptase RT PCR Master Mix (5X) |
|||
| MB1002-25reactions | BosterBio | 25 reactions | EUR 226.8 |
Powerpol HS 2X PCR Mix for Mouse Genotyping |
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| RK20737 | Abclonal | 1.250mL | EUR 134 |
Extra T7 gRNA PCR primer mix (5 uM) |
|||
| CAS510A-PR | SBI | 50 assays | EUR 99 |
Taq 5x PCR Mix Ready-to-Load 12.5 mM |
|||
| BT10502 | Bioatlas | 250rxn | EUR 136.8 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
|||
N-Flux™ 5X Digital PCR Master Mix |
|||
| 31078 | Biotium | 1 | EUR 443 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
|||
N-Flux™ 5X Digital PCR Master Mix |
|||
| 31078-T | Biotium | 1 | EUR 99 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
|||
ReadiUseâ„¢ dNTP Mix Set *10 mM PCR Grade* |
|||
| 17258-1mL | AAT Bioquest | 1 mL | EUR 79 |
|
Description: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. |
|||
Original INTELLI-PLATE 96-2; 2-well sitting drop, 120 plates |
|||
| MAR-102-0011-00 | MiTeGen | 120 PLATES | EUR 990 |
|
Description: Original INTELLI-PLATE 96-2; 2-well sitting drop, 120 plates |
|||
2x Genotyping PCR Ready Master Mix (250 x 20µL rxn) |
|||
| T403M | 101Bio | 125 rxn | EUR 99 |
2x Genotyping PCR Ready Master Mix (250 x 20µL rxn) |
|||
| T403S | 101Bio | 50 rxn | EUR 49 |
2x Genotyping PCR Ready Master Mix (250 x 20µL rxn) |
|||
| T403 | 101Bio | - | Ask for price |
ReadiUse™ dNTP Mix Set *10 mM PCR Grade* |
|||
| 17258 | AAT Bioquest | 1 mL | EUR 79 |
INTELLI-PLATE 96-2 flat bottom; 2-well sitting drop, 120 plates |
|||
| MAR-102-0001-01 | MiTeGen | 120 PLATES | EUR 990 |
|
Description: INTELLI-PLATE 96-2 flat bottom; 2-well sitting drop, 120 plates |
|||
INTELLI-PLATE 96-2 low profile; 2-well sitting drop, 120 plates |
|||
| MAR-102-0001-10 | MiTeGen | 120 PLATES | EUR 990 |
|
Description: INTELLI-PLATE 96-2 low profile; 2-well sitting drop, 120 plates |
|||
INTELLI-PLATE 96-3 low profile; 3-well sitting drop, 120 plates |
|||
| MAR-102-0001-13 | MiTeGen | 120 PLATES | EUR 990 |
|
Description: INTELLI-PLATE 96-3 low profile; 3-well sitting drop, 120 plates |
|||
Apparently, solely 3-5% of the oligo-DNA strands on the dsDNA-PS particles fashioned a duplex with complementary DNAs, and the F-D curves confirmed a definite specificity to the duplex terminal sequences within the interparticle drive at a high-NaCl focus; a transparent attraction peak was noticed in F-D curves solely when the duplex terminal was a complementary base pair.