Vibrio cholerae infects human hosts following ingestion of contaminated meals or water, ensuing within the extreme diarrheal illness cholera. The watery diarrhea that’s attribute of the illness is immediately brought on by manufacturing of cholera toxin (CT). A fancy regulatory cascade controls manufacturing of CT and different virulence elements. Nevertheless, finally a single protein, ToxT, immediately binds to virulence gene promoters and prompts their transcription. Beforehand, we recognized two ToxT binding websites, or toxboxes, inside the cholera toxin promoter (PctxAB). The toxboxes overlap with the 2 promoter-proximal GATTTTT heptad repeats discovered inside PctxAB in classical biotype V. cholerae pressure O395.
These heptad repeats had been beforehand discovered to be inside a big DNA area sure by H-NS, a worldwide transcriptional repressor current in Gram-negative micro organism. The present mannequin for management of PctxAB transcription proposed full H-NS displacement from the DNA by ToxT, adopted by direct activation by ToxT-RNAP contacts. The aim of this examine was to find out extra exactly the place H-NS binds to PctxAB and check the speculation that ToxT fully displaces H-NS from the PctxAB promoter earlier than activating transcription.
Outcomes recommend that H-NS binds solely to the area of PctxAB encompassing the heptad repeats and ToxT solely displaces H-NS from its most promoter proximal binding websites, calling for a revision of the present mannequin involving H-NS and ToxT at PctxAB. H-NS is a worldwide detrimental regulator of transcription in Gram detrimental micro organism, significantly in horizontally acquired genetic islands. Earlier work in Vibrio cholerae recommended that H-NS represses transcription of cholera toxin genes by binding to a big area upstream of its promoter, and that virulence activator ToxT derepresses transcription by eradicating H-NS from the promoter.
Right here, new knowledge assist a revised mannequin wherein ToxT solely displaces H-NS sure to essentially the most promoter proximal DNA websites that overlap the ToxT binding websites, leaving the upstream websites occupied by H-NS. This introduces the next decision mechanism for antirepression of H-NS in charge of cholera toxin manufacturing.
Growth of DNA Markers From Bodily Mapped Loci in Aegilops comosa and Aegilops umbellulata Utilizing Single-Gene FISH and Chromosome Sequences
Breeding of agricultural crops tailored to local weather change and proof against ailments and pests is hindered by a restricted gene pool due to domestication and hundreds of years of human choice. One approach to enhance genetic variation is chromosome-mediated gene switch from wild family by cross hybridization. Within the case of wheat (Triticum aestivum), the species of genus Aegilops are a very enticing supply of latest genes and alleles. Nevertheless, through the evolution of the Aegilops and Triticum genera, diversification of the D-genome lineage resulted within the formation of diploid C, M, and U genomes of Aegilops.
The extent of structural genome alterations, which accompanied their evolution and speciation, and the scarcity of molecular instruments to detect Aegilops chromatin hamper gene switch into wheat. To analyze the chromosome construction and assist develop molecular markers with a identified bodily place that might enhance the effectivity of the choice of desired introgressions, we developed single-gene fluorescence in situ hybridization (FISH) maps for M- and U-genome progenitors, Aegilops comosa and Aegilops umbellulata, respectively. Forty-three ortholog genes had been situated on 47 loci in Ae. comosa and on 52 loci in Ae. umbellulata utilizing wheat cDNA probes.
The outcomes obtained confirmed that M-genome chromosomes preserved collinearity with these of wheat, excluding 2 and 6M containing an intrachromosomal rearrangement and paracentric inversion of 6ML, respectively. Whereas Ae. umbellulata chromosomes 1, 3, and 5U maintained collinearity with wheat, structural reorganizations in 2, 4, 6, and 7U recommended a similarity with the C genome of Aegilops markgrafii. To develop molecular markers with precise bodily positions on chromosomes of Aegilops, the single-gene FISH knowledge had been validated in silico utilizing DNA sequence assemblies from flow-sorted M- and U-genome chromosomes.
The sequence similarity search of cDNA sequences confirmed 44 out of the 47 single-gene loci in Ae. comosa and 40 of the 52 map positions in Ae. umbellulata. Polymorphic areas, thus, recognized enabled the event of molecular markers, which had been PCR validated utilizing wheat-Aegilops disomic chromosome addition traces. The one-gene FISH-based strategy allowed the event of PCR markers particular for cytogenetically mapped positions on Aegilops chromosomes, substituting as but unavailable segregating map. The brand new information and assets will assist the efforts for the introgression of Aegilops genes into wheat and their cloning.
Terminal Sequence-Particular Interparticle Attraction between DNA Duplex-Carrying Polystyrene Microparticles in Aqueous Salt Answer Assessed by Optical Tweezers
The dispersion conduct of DNA duplex-carrying colloidal particles in aqueous high-salt options exhibits extraordinary selectivity in opposition to the duplex terminal sequence. We investigated the interparticle drive between DNA duplex-carrying polystyrene (dsDNA-PS) microparticles in aqueous salt options and examined their conduct in relation to the duplex terminal sequences. Drive-distance (F-D) curves for a pair of dsDNA-PS particles had been recorded with a dual-beam optical tweezers system with the 2 optically trapped particles intently approaching one another.
Volatile Organics Mix |
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| 60-BIG-MIX | Scientific Laboratory Supplies | 1ML | EUR 120.84 |
2×Taq PCR Master Mix(with dye) |
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| K1034-1 | ApexBio | 1 ml | EUR 87.6 |
Semi-volatile Organics Mix |
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| 76-BIG-MIX | Scientific Laboratory Supplies | 1ML | EUR 150.48 |
Ready? PCR Mix |
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| M1127-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix |
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| M1127-200 | Biovision | each | EUR 229.2 |
Rigor? PCR Mix |
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| M1132-200 | Biovision | each | EUR 385.2 |
Breeze? PCR Mix |
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| M1134-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix |
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| M1136-200 | Biovision | each | EUR 352.8 |
Advance? PCR Mix |
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| M1139-200 | Biovision | each | EUR 451.2 |
Volatile Organics Mix (76 Components) |
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| 8260-BIG-MIX | Scientific Laboratory Supplies | 1ML | EUR 296.4 |
Methylamp PCR Enhancer |
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| R-1002 | EpiGentek |
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Egg Drop Syndrome RT PCR kit |
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| RTq-V026-100D | Bioingentech | 100T | EUR 860.4 |
Egg Drop Syndrome RT PCR kit |
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| RTq-V026-150D | Bioingentech | 150T | EUR 969.6 |
Egg Drop Syndrome RT PCR kit |
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| RTq-V026-50D | Bioingentech | 50T | EUR 717.6 |
lyophilised real-time PCR Master Mix for dual labeled probes |
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| M-PCR-156L | MiTeGen | 960 reactions x 20 µl | EUR 900.68 |
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Description: lyophilised real-time PCR Master Mix for dual labeled probes |
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lyophilised real-time PCR Master Mix for dual labeled probes |
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| M-PCR-156S | MiTeGen | 192 reactions x 20 µl | EUR 225.3 |
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Description: lyophilised real-time PCR Master Mix for dual labeled probes |
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Individual Reaction Mix 1 |
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| G065-1 | ABM | 200 reactions | EUR 200.4 |
dNTP Mix (1 ml) |
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| 9002-1 | Biovision | each | EUR 183.6 |
PCR-EZ D-PCR MASTER MIX |
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| BS294 | Bio Basic | 100RXN, 100prep | EUR 112.2 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-100D | Bioingentech | 100T | EUR 1039.2 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-150D | Bioingentech | 150T | EUR 1177.2 |
Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-50D | Bioingentech | 50T | EUR 861.6 |
amfiSure PCR Master Mix |
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| P0311-010 | GenDepot | 2x50 rxns | EUR 151.2 |
amfiSure PCR Master Mix |
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| P0311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure PCR Master Mix |
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| P0311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure PCR Master Mix |
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| P0311-100 | GenDepot | 10x1ml | EUR 343.2 |
amfiSure PCR Master Mix |
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| P0311-125 | GenDepot | 15X50 rxns | EUR 333.6 |
amfiSure PCR Master Mix |
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| P0311-200 | GenDepot | 20x1ml | EUR 562.8 |
amfiSure PCR Master Mix |
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| P0311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure PCR Master Mix |
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| P0311-500 | GenDepot | 100x50 rxns | EUR 1509.6 |
amfiXpand PCR Master Mix |
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| P0331-010 | GenDepot | 2X50 rxns | EUR 181.2 |
amfiXpand PCR Master Mix |
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| P0331-025 | GenDepot | 5X50 rxns | EUR 327.6 |
amfiXpand PCR Master Mix |
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| P0331-050 | GenDepot | 10X50 rxns | EUR 537.6 |
amfiXpand PCR Master Mix |
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| P0331-250 | GenDepot | 50x50 rxns | EUR 2247.6 |
Ready? PCR Mix-Dye |
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| M1128-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix-Dye |
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| M1128-200 | Biovision | each | EUR 229.2 |
Image Ready? PCR Mix |
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| M1129-200 | Biovision | each | EUR 352.8 |
Robust Ready? PCR Mix |
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| M1130-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix |
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| M1130-200 | Biovision | each | EUR 255.6 |
Rigor? PCR Mix-Dye |
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| M1133-200 | Biovision | each | EUR 385.2 |
Breeze? PCR Mix-Dye |
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| M1135-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix-Dye |
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| M1137-200 | Biovision | each | EUR 352.8 |
Image Distant? PCR Mix |
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| M1138-200 | Biovision | each | EUR 385.2 |
Advance? PCR Mix-Dye |
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| M1140-200 | Biovision | each | EUR 451.2 |
Fire Start? PCR Mix |
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| M1141-200 | Biovision | each | EUR 451.2 |
Whole Blood PCR Mix |
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| M1143-200 | Biovision | each | EUR 385.2 |
Volatile Organics Mix (Low Level) |
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| 60-BIG-MIX-200 | Scientific Laboratory Supplies | 1ML | EUR 116.28 |
Volatile Organics Mix (High Level) |
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| 60-BIG-MIX-2000 | Scientific Laboratory Supplies | 1ML | EUR 165.3 |
2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn |
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| W0655-1 | 101Bio | - | Ask for price |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-100D | Bioingentech | 100T | EUR 860.4 |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-150D | Bioingentech | 150T | EUR 969.6 |
The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-50D | Bioingentech | 50T | EUR 717.6 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 789.36 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 197.34 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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PRICE DROP |
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| 110-012 | GeneOn | 4 x 1000 µl | EUR 159.6 |
PRICE DROP |
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| 110-012L | GeneOn | 5x 4 x 1000 µl | EUR 621.6 |
PRICE DROP |
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| 110-012XL | GeneOn | 10x 4 x 1000 µl | EUR 1126.8 |
MyTaq Blood PCR Mix, 2x |
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| BIO-25053/S | Bioline | Sample | Ask for price |
MyTaq Blood PCR Mix, 2x |
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| BIO-25054 | Bioline | 250 Reactions | Ask for price |
mRNAExpress Tail PCR mix (5uM) |
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| MR-TAIL-PR | SBI | 100 ul | EUR 196.8 |
amfiSure Prime PCR Master Mix |
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| P1311-025 | GenDepot | 5X50 rxns | EUR 193.2 |
amfiSure Prime PCR Master Mix |
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| P1311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure Prime PCR Master Mix |
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| P1311-125 | GenDepot | 15X50 rxns | EUR 327.6 |
amfiSure Prime PCR Master Mix |
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| P1311-500 | GenDepot | 100x50 rxns | EUR 1485.6 |
amfiSure Advanced PCR Master Mix |
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| P2311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure Advanced PCR Master Mix |
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| P2311-050 | GenDepot | 10X50 rxns | EUR 267.6 |
amfiSure Advanced PCR Master Mix |
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| P2311-125 | GenDepot | 15X50 rxns | EUR 338.4 |
amfiSure Advanced PCR Master Mix |
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| P2311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure Advanced PCR Master Mix |
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| P2311-500 | GenDepot | 100x50 rxns | EUR 1534.8 |
Robust Ready? PCR Mix-Dye |
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| M1131-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix-Dye |
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| M1131-200 | Biovision | each | EUR 255.6 |
Fire Start? PCR Mix-Dye |
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| M1142-200 | Biovision | each | EUR 451.2 |
TuMinute PCR Clean-Up Kit |
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| P-1005 | EpiGentek |
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AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE) |
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| MAG-PCR-NM-1 | CORNING | 1/pk | EUR 87.6 |
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Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
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The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-100D | Bioingentech | 100T | EUR 1039.2 |
The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-150D | Bioingentech | 150T | EUR 1177.2 |
The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-50D | Bioingentech | 50T | EUR 861.6 |
AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE) |
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| MAG-PCR-CL-1 | CORNING | 1/pk | EUR 69.6 |
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Description: Bioscience Mag Beads; Magnetic PCR Clean Up |
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PCR Tubes (50) |
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| PCR-50 | BioAssay Systems | 50 | EUR 15 |
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Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes. |
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Bluetongue virus 1 PCR kit |
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| PCR-V226-48R | Bioingentech | 50T | EUR 987.6 |
Bluetongue virus 1 PCR kit |
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| PCR-V226-96R | Bioingentech | 100T | EUR 1335.6 |
Poliovirus 1 (PV1) PCR kit |
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| PCR-H659-48R | Bioingentech | 50T | EUR 987.6 |
Poliovirus 1 (PV1) PCR kit |
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| PCR-H659-96R | Bioingentech | 100T | EUR 1335.6 |
Psittacid herpesvirus 1 PCR kit |
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| PCR-V014-48D | Bioingentech | 50T | EUR 543.6 |
Psittacid herpesvirus 1 PCR kit |
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| PCR-V014-96D | Bioingentech | 100T | EUR 686.4 |
Equine herpesvirus 1 PCR kit |
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| PCR-V144-48D | Bioingentech | 50T | EUR 543.6 |
Equine herpesvirus 1 PCR kit |
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| PCR-V144-96D | Bioingentech | 100T | EUR 686.4 |
Bovine herpesvirus 1 PCR kit |
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| PCR-V182-48D | Bioingentech | 50T | EUR 543.6 |
Bovine herpesvirus 1 PCR kit |
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| PCR-V182-96D | Bioingentech | 100T | EUR 686.4 |
Taq 2x PCR Mix 100 rxn |
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| BA01501 | Bioatlas | 100rxn | EUR 112.8 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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HotTaq 2x PCR Mix 100 rxn |
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| BA01503 | Bioatlas | 100rxn | EUR 144 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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RedTaq 2x PCR Mix 100 rxn |
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| BA01507 | Bioatlas | 100rxn | EUR 175.2 |
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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Taq 5x PCR Mix 12.5 mM |
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| BT10402 | Bioatlas | 250rxn | EUR 130.8 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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amfiSure ONE PCR Master Mix(2X) |
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| P7000-005 | GenDepot | 5x1 ml | EUR 262.8 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-010 | GenDepot | 10x1 ml | EUR 380.4 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-050 | GenDepot | 50x1 ml | EUR 1453.2 |
amfiSure ONE PCR Master Mix(2X) |
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| P7000-100 | GenDepot | 100x1 ml | EUR 2522.4 |
qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-360L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-360S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-362L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-362S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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Pesticide Mix 1 Containing 16 Compounds in Acetonitrile |
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| SPXPR-1 | Scientific Laboratory Supplies | 1ML | EUR 282.72 |
qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-361L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-361S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-363L | MiTeGen | 10 x 1,25 ml | EUR 864.5 |
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Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-363S | MiTeGen | 2 x 1,25 ml | EUR 216.19 |
|
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
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| M-PCR-321L | MiTeGen | 10 x 1,25 ml | EUR 1438.81 |
|
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
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| M-PCR-321S | MiTeGen | 2 x 1,25 ml | EUR 359.77 |
|
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
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MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-48D | Bioingentech | 50T | EUR 543.6 |
MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-96D | Bioingentech | 100T | EUR 686.4 |
Caprine herpes virus 1 PCR kit |
|||
| PCR-V312-48D | Bioingentech | 50T | EUR 543.6 |
Caprine herpes virus 1 PCR kit |
|||
| PCR-V312-96D | Bioingentech | 100T | EUR 686.4 |
Mouse parvovirus type 1 PCR kit |
|||
| PCR-V324-48D | Bioingentech | 50T | EUR 543.6 |
Mouse parvovirus type 1 PCR kit |
|||
| PCR-V324-96D | Bioingentech | 100T | EUR 686.4 |
Borna disease viruses 1 PCR kit |
|||
| PCR-V605-48R | Bioingentech | 50T | EUR 987.6 |
Borna disease viruses 1 PCR kit |
|||
| PCR-V605-96R | Bioingentech | 100T | EUR 1335.6 |
Herpes simplex type 1 PCR kit |
|||
| PCR-H426-48D | Bioingentech | 50T | EUR 543.6 |
Herpes simplex type 1 PCR kit |
|||
| PCR-H426-96D | Bioingentech | 100T | EUR 686.4 |
Dengue virus type 1 PCR kit |
|||
| PCR-H521-48R | Bioingentech | 50T | EUR 987.6 |
Dengue virus type 1 PCR kit |
|||
| PCR-H521-96R | Bioingentech | 100T | EUR 1335.6 |
Canine adenovirus type 1 PCR kit |
|||
| PCR-V077-48D | Bioingentech | 50T | EUR 543.6 |
Canine adenovirus type 1 PCR kit |
|||
| PCR-V077-96D | Bioingentech | 100T | EUR 686.4 |
qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-364L | MiTeGen | 10 x 1,25 ml | EUR 864.5 |
|
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-364S | MiTeGen | 2 x 1,25 ml | EUR 216.19 |
|
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-365L | MiTeGen | 10 x 1,25 ml | EUR 864.5 |
|
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-365S | MiTeGen | 2 x 1,25 ml | EUR 216.19 |
|
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
Canadian Pesticide Mix 1 Containing 14 Compounds |
|||
| CAN-CAN-1 | Scientific Laboratory Supplies | 1ML | EUR 98.04 |
qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-322L | MiTeGen | 10 x 1,25 ml | EUR 1438.81 |
|
Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-322S | MiTeGen | 2 x 1,25 ml | EUR 359.77 |
|
Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-323L | MiTeGen | 10 x 1,25 ml | EUR 1438.81 |
|
Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-323S | MiTeGen | 2 x 1,25 ml | EUR 359.77 |
|
Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|||
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
|||
| PCR-MPX401-48D | Bioingentech | 50T | EUR 543.6 |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
|||
| PCR-MPX401-96D | Bioingentech | 100T | EUR 686.4 |
Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-396L | MiTeGen | 10 x 1,25 ml | EUR 900.05 |
|
Description: Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|||
| M-PCR-396S | MiTeGen | 2 x 1,25 ml | EUR 225.04 |
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Description: Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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Smart 2X PCR pre-mix Pfu (30ul) |
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| 9K-002-0038 | Bio Basic | 30uL, 30uL | EUR 697.37 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | EUR 251.4 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | EUR 115.68 |
Taq PCR Master Mix (2X, Blue Dye) |
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| BS9295 | Bio Basic | 1ml | EUR 101.76 |
Taq PCR Master Mix (2X, Blue Dye) |
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| BS9296 | Bio Basic | 5ml | EUR 227.04 |
Taq PCR Master Mix (2X, Red Dye) |
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| BS9297 | Bio Basic | 1ml | EUR 101.76 |
Taq PCR Master Mix (2X, Red Dye) |
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| BS9298 | Bio Basic | 5ml | EUR 227.04 |
Reverse Transcriptase RT PCR Master Mix (5X) |
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| MB1002-100reactions | BosterBio | 100 reactions | EUR 616.8 |
Reverse Transcriptase RT PCR Master Mix (5X) |
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| MB1002-200reactions | BosterBio | 200 reactions | EUR 895.2 |
Apparently, solely 3-5% of the oligo-DNA strands on the dsDNA-PS particles fashioned a duplex with complementary DNAs, and the F-D curves confirmed a definite specificity to the duplex terminal sequences within the interparticle drive at a high-NaCl focus; a transparent attraction peak was noticed in F-D curves solely when the duplex terminal was a complementary base pair.