Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection
Nucleobase mismatches can jeopardize DNA polymerization specificity, inflicting mutations and errors in DNA replication and detection. Herein we report the primary synthesis of novel 2-Se-thymidine triphosphate (SeTTP), describe the single-selenium atom-specific modification technique (SAM) towards T/G mismatches, and exhibit SAM-assisted polymerization and detection with a lot greater specificity and sensitivity.
SAM can successfully suppress the formation of non-specific merchandise in DNA polymerization and detection. Thus, SAM enhances the specificity of DNA synthesis by roughly 10 000 fold, and in flip, it permits the detection of scientific COVID-19 viral RNA in low copy numbers (single-digit copies), whereas the standard RT-qPCR doesn’t.
Remedy of Triple Unfavourable Cell Traces with Olaparib to Block DNA Restore
A Versatile One-Step Aggressive Fiber Optic Floor Plasmon Resonance Bioassay Enabled by DNA Nanotechnology
- On this method, we enabled one-step aggressive detection and will obtain vital alerts, unbiased of the burden of the goal molecules, with out requiring labeling or preprocessing steps. This was demonstrated for the detection of a small molecule (ATP), protein (thrombin), and ssDNA goal, thereby reaching detection limits of 72 μM, 36 nM, and 30 nM respectively and proving the generalizability of the proposed bioassay.
- Moreover, goal detection was efficiently achieved in 10-fold diluted plasma, which demonstrated the applicability of the assay in biologically related matrices. Altogether, the developed one-step aggressive FO-SPR bioassay opens up prospects for the detection of low-molecular-weight targets in a quick and simple method.
A PCR-amplified transgene fragment flanked by a single copy of a truncated inverted terminal repeat for recombinant adeno-associated virus manufacturing prevents pointless plasmid DNA packaging
Recombinant Salmonella typhi DNA-binding protein HU-beta (hupB) |
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RPC20489-20ug | Biomatik Corporation | 20ug | EUR 448.1 |
Recombinant Salmonella typhi flag.. [His] |
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DAGA-3084 | Creative Diagnostics | 1mg | EUR 2574 |
Recombinant Salmonella typhi OMP protein |
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DAG-S001 | Creative Diagnostics | 1 mg | EUR 1992 |
Salmonella Typhi H Antigen Recombinant |
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rAP-5461 | Angio Proteomie | Inquiry | Ask for price |
Salmonella Typhi Haemolysin E Recombinant |
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rAP-5464 | Angio Proteomie | Inquiry | Ask for price |
Recombinant Salmonella Typhi S. Typhi H Protein, His, E.coli-1mg |
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QP13382-1mg | EnQuireBio | 1mg | EUR 1513.2 |
Recombinant Salmonella Typhi S. Typhi OMP Protein, His, E.coli-1mg |
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QP13384-1mg | EnQuireBio | 1mg | EUR 1513.2 |
Recombinant Salmonella typhi pagC [His], 24 kD |
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DAGA-247 | Creative Diagnostics | 1 mg | EUR 2401.2 |
Recombinant Salmonella Typhi S. Typhi H Protein, His, E.coli-100ug |
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QP13382-100ug | EnQuireBio | 100ug | EUR 261.6 |
Recombinant Salmonella Typhi S. Typhi H Protein, His, E.coli-500ug |
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QP13382-500ug | EnQuireBio | 500ug | EUR 795.6 |
Recombinant Salmonella Typhi S. Typhi HylE Protein, His, E.coli-1mg |
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QP13383-1mg | EnQuireBio | 1mg | EUR 795.6 |
Recombinant Salmonella Typhi S. Typhi OMP Protein, His, E.coli-100ug |
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QP13384-100ug | EnQuireBio | 100ug | EUR 261.6 |
Recombinant Salmonella Typhi S. Typhi OMP Protein, His, E.coli-500ug |
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QP13384-500ug | EnQuireBio | 500ug | EUR 795.6 |
Recombinant Salmonella Typhi S. Typhi HylE Protein, His, E.coli-100ug |
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QP13383-100ug | EnQuireBio | 100ug | EUR 241.2 |
Recombinant Salmonella Typhi S. Typhi HylE Protein, His, E.coli-500ug |
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QP13383-500ug | EnQuireBio | 500ug | EUR 555.6 |
Recombinant Salmonella Typhi S. Typhi OMP 52kDa Protein, His, E.coli-1mg |
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QP13385-1mg | EnQuireBio | 1mg | EUR 795.6 |
Recombinant Salmonella Typhi S. Typhi OMP 52kDa Protein, His, E.coli-100ug |
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QP13385-100ug | EnQuireBio | 100ug | EUR 241.2 |
Recombinant Salmonella Typhi S. Typhi OMP 52kDa Protein, His, E.coli-500ug |
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QP13385-500ug | EnQuireBio | 500ug | EUR 555.6 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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CSB-EP676401CBAB | Cusabio | 3909 mg | Ask for price |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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CSB-EP676401CBABe1 | Cusabio | 3910 mg | Ask for price |
Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB) |
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AP77378 | SAB | 1mg | EUR 3015 |
Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB) |
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AP77379 | SAB | 1mg | EUR 2826 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23107-100ug | Biomatik Corporation | 100ug | EUR 721.6 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23107-1mg | Biomatik Corporation | 1mg | EUR 2884.7 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23107-20ug | Biomatik Corporation | 20ug | EUR 448.1 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23108-100ug | Biomatik Corporation | 100ug | EUR 735.9 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23108-1mg | Biomatik Corporation | 1mg | EUR 3078.4 |
Recombinant Colwellia psychrerythraea DNA polymerase IV (dinB) |
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RPC23108-20ug | Biomatik Corporation | 20ug | EUR 519.2 |