Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

Anomopoda is the widespread planktonic microcrustacean, which performs a vital function in aquatic ecosystem. There are few research in regards to the evolutionary relationships amongst varied Anomopoda basing on molecular information. Within the current examine, phylogenetic evaluation of eight Anomopoda was carried out. Firstly, the tradition system was developed to breed cladocerans. Through the use of this technique, eight species (Daphnia magna, D. pulex, D. sinensis, Ceriodaphnia reticulata, Moina micrura, Scapholeberis kingi, Simocephalus vetulus and Eurycercus lamellatus) had been purified and cultured stably within the laboratory.

Then, transcriptomic sequences and partial mitochondrial DNA sequences had been each used to reconstruct the phylogenetic tree amongst eight species. Transcriptomic sequences had been sequenced on Illumina Hiseq 2500 platform. After meeting and annotation, transcriptomic sequences had been spliced collectively and aligned for phylogenetic evaluation. Basing on the orthologous genes derived from transcriptomic sequences, the phylogenetic evaluation confirmed that four genera of Daphniidae had been clustered into one group, and among the many four genera, Ceriodaphnia was nearer to Daphnia than Simocephalus, whereas Scapholeberis was farthest from different species. As well as, Eurycercidae was nearer to Daphniidae than Moinidae.

The phylogenetic bushes primarily based on each 12S rRNA and 16S rRNA sequences had been related with that primarily based on transcriptomic sequences. In the meantime, the phylogenetic tree primarily based on 16S rRNA sequences was extra appropriate than that primarily based on 12S rRNA sequences. These outcomes steered that the phylogenetic evaluation basing on the transcriptomic sequences was accessible in cladocerans, which can assist us to successfully perceive the phylogenetic relationships amongst varied cladocerans.

Utility of DNA sequences in anti-counterfeiting: Present progress and challenges

Counterfeiting has by no means been tougher than in the course of the COVID-19 pandemic as counterfeit take a look at kits and therapeutics have been found out there. Present anti-counterfeiting labels have weaknesses: they will both be duplicated simply, are costly or ill-suited for the prevailing advanced provide chains. Whereas RFID tags present for a wonderful different to present anti-counterfeiting strategies, they will show to be costly and different routes involving nanomaterials will be troublesome to encrypt.

A DNA primarily based anticounterfeiting system has vital benefits comparable to relative ease of synthesis and huge information storage skills, together with nice potential in encryption. Though DNA is provided with such helpful properties, main challenges that restrict its real-world anti-counterfeiting functions embody safety in harsh environments, speedy cheap sequence dedication, and its attachment to merchandise. This examine was performed on 62 soil samples collected from livestock stables. The samples had been cultured by way of hair bait approach (HBT).

This assessment elaborates the present progress of DNA primarily based anti-counterfeiting programs and identifies technological gaps that must be stuffed for its sensible utility. Progress made on addressing the first challenges related to the usage of DNA, and potential options are mentioned. Keratinophilic fungi play an vital function within the decomposition of keratinous substances in nature. This capability induces dermatomycosis in each people and livestock. The soil of livestock stables could be a reservoir of keratinophilic fungi. Subsequently, the current examine was performed to isolate and determine keratinophilic fungi within the soil of the livestock stables positioned in Qayen, South Khorasan Province, Iran.

Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

The impression of flanking sequence options on DNA CpG methylation

Epigenetics and DNA methylation play a pivotal function in lots of processes of the cell and we regularly observe that an aberrant methylation sample characterizes pathologies. On this work we examine the function that the flanking sequences of CGs play within the methylation course of in human. We constructed 4 totally different CG datasets: methylated, unmethylated, and two randomly extracted ones. We evaluated options related to the flanking sequences of these CG units, for various measurement across the CG, via 5 measures accounting for various features of sequence composition complexity and construction.

The evaluation carried out via these measures revealed evident totally different behaviors between methylated and unmethylated probe units. Main variations had been noticed for GC content material and CG dinucleotide frequency in a window measurement of 300-400 bp and for CG self-attraction in 3K bp. It’s exceptional because the impact of methylated CG lasts far more than anticipated removed from the CG.

Full mitochondrial DNA sequence of Norwegian skates ( Raja brachyuran Lafont, 1871) imported to Korea

On this examine, the entire mitochondrial genome of Norwegian skates imported to Korea was sequenced with a round molecule of 17,121 bp, which consisted of 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 switch RNA genes, and a management area (D-loop). And amongst these sequences, 193 bp sequence within the D-loop of the genus Raja steered the opportunity of getting used as a genetic marker for classification of Raja and Dipturus species.

The BI phylogenetic tree by utilizing the nucleotide sequences of 13 PCGs from 15 accessible mitogenomes of household Rajidae confirmed additionally that Norwegian skates imported to Korea kind a bunch with Raja brachyura species with excessive department worth, and that this was a species of Raja brachyura. As above, these outcomes could be anticipated to supply for the additional understanding on the phylogenetic relationship, taxonomic classification and phylogeography of the household Rajidae.

6 kb plus DNA Ladder

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1 kb DNA Ladder 500ul per kit

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EUR 148.8

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
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Description: Bioscience Mol Bio; DNA Ladder

ReadiUseâ„¢ 1 Kb Plus DNA Ladder

60050-100ul 100 ul
EUR 99
Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

ReadiUseâ„¢ 1 Kb Plus DNA Ladder

60051-2X250ul 2X250 ul
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Description: ReadiUseâ„¢ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder

60055-100ul 100 ul
EUR 79
Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

ReadiUseâ„¢ GeneRuler 1 kb DNA Ladder

60056-2X250ul 2X250 ul
EUR 151
Description: ReadiUseâ„¢ GeneRuler 1 kb DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

1 kb DNA Ladder in TE buffer

31039 300uL
EUR 169.2
Description: Minimum order quantity: 1 unit of 300uL

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
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Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
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50 kb large-Range DNA ladder

308-025 250 µg
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DNA LADDER (100bp)

IB01301 50uG/500uL
EUR 59.65
Description: [FR]

DNA LADDER (100bp)

IB01302 50uG/500uL
EUR 56.1
Description: (NO LOADING DYE) [FR]

DNA LADDER (1KB)

IB01351 50uG/500uL
EUR 61.65
Description: [FR]

100bp DNA ladder

9K-004-0002 0.5ml
EUR 260.98

100 bp DNA Ladder

M1158-500 each
EUR 333.6

100 bp DNA Ladder

MD104-01 250 μl
EUR 132

100 bp DNA Ladder

MD104-02 500 μl
EUR 139.2

100 bp DNA Ladder

RM19007 500μL
EUR 138

ACTGene? DNA Marker, 1 kb Ladder, 13 Fragments, 100 loads

ACT-IDMWD1 each
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*Low Range DNA Ladder

IDNALDRLEL500UL each
EUR 157
Description: *Low Range DNA Ladder

*Mid Range DNA Ladder

IDNALDRMEL500UL each
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Description: *Mid Range DNA Ladder

iVDye 1Kb DNA Ladder

V1003-001 1ml
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iVDye 1Kb DNA Ladder

V1003-025 250ul
EUR 91.2

iVDye 1Kb DNA Ladder

V1003-100 4x250ul
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iVDye 1Kb DNA Ladder

V1003-250 10x250ul
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iVDye 1Kb DNA Ladder

V1003-500 20x250ul
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*High Range DNA Ladder

IDNALDRHEL500UL each
EUR 105
Description: *High Range DNA Ladder

iVDye 50bp DNA Ladder

V1001-001 1ml
EUR 218.4

iVDye 50bp DNA Ladder

V1001-025 250ul
EUR 105.6

iVDye 50bp DNA Ladder

V1001-100 4x250ul
EUR 230.4

iVDye 50bp DNA Ladder

V1001-250 10X250ul
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iVDye 100bp DNA Ladder

V1002-001 1ml
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iVDye 100bp DNA Ladder

V1002-025 250ul
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iVDye 100bp DNA Ladder

V1002-100 4x250ul
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iVDye 100bp DNA Ladder

V1002-250 10X250ul
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D2000 plus DNA Ladder

RM19004 500μL
EUR 138

OneMark B DNA Ladder (ready-to-use, 250-1000 kb)

DM110-0100 600 µl
EUR 75.6

XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes)

300007 50µg/500µl
EUR 84

XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes)

300008 5x50 µg
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ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads

ACT-IDMWD2 each
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ReadiUseâ„¢ 100 bp DNA Ladder

60070-100ul 100 ul
EUR 99
Description: ReadiUseâ„¢ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

ReadiUseâ„¢ 100 bp DNA Ladder

60071-2X250ul 2X250 ul
EUR 203
Description: ReadiUseâ„¢ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

100 bp Ladder DNA Marker

FYD002-500UL 30 µg/ 500 µl Ask for price

Star 1kb DNA Ladder Express

BT10701 500µl
EUR 96
Description: High purity buffer for various PCR applications.

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300005 86µg/500µl
EUR 84

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006 5x86 µg
EUR 253.2

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006L 10x86 µg
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One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006XL 20x86 µg
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50 bp Ladder DNA Marker

FYD004-500UL 32.5 µg/ 500 µl Ask for price

100 bp DNA Ladder 500ul per k

AXY1014 EACH
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100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

Wide range 100 bp DNA Ladder

M1192-500 each
EUR 333.6

Star 100 bp DNA Ladder Express

BA01302 500µl
EUR 96
Description: High purity buffer for various PCR applications.

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
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Apoptotic DNA Ladder Detection Kit

EBA15100 100 assays
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Apoptotic DNA Ladder Detection Kit

EBA1520 20 assays
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Apoptotic DNA Ladder Detection Kit

EBA1550 50 assays
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20 bp TinyOrange DNA Ladder (105ng/µl)

309-005 500 µl
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Azura PureView 250bp DNA Ladder - 1000 Lanes

AZ-1125-2 1000 Lanes
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AZ-1135-2 1000 Lanes
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Azura PureView 50bp DNA Ladder - 1000 Lanes

AZ-1155-2 1000 Lanes
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Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
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Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
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Azura PureView 100bp DNA Ladder - 100 Lanes

AZ-1131 100 Lanes
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Azura PureView 100bp DNA Ladder - 500 Lanes

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Azura PureView 50bp DNA Ladder - 100 Lanes

AZ-1151 100 Lanes
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Azura PureView 50bp DNA Ladder - 500 Lanes

AZ-1155 500 Lanes
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1000bp/1kb DNA ladder (no loading dye)

305-005 50µg/250µl
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1000bp/1kb DNA ladder (no loading dye)

305-025 5x50µg
EUR 144

Quick Apoptotic DNA Ladder Detection Kit

K120-50 each
EUR 548.4

Quick Apoptotic DNA Ladder Detection Kit

K2194-50 50 assays
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1Kb Plus DNA Ladder, Ready-to-use

9K-004-0001 0.5ml
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Enhanced Apoptotic DNA Ladder Detection Kit

K130-50 each
EUR 529.2

Enhanced Apoptotic DNA Ladder Detection Kit

K2202-50 50 assays
EUR 568.8

100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye)

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Accuris SmartCheck™ 1kb DNA Ladder, 500ul / 100 lanes

PR4100-100 1 PC
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Accuris SmartCheck™ 50bp DNA Ladder, 500ul / 100 lanes

PR4005-100 1 PC
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Accuris SmartCheck™ 100bp DNA Ladder, 500ul / 100 lanes

PR4010-100 1 PC
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1000bp/1kb DNA ladder (blue, ready-to-use)

305-105 50µg/500µl
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1000bp/1kb DNA ladder (blue, ready-to-use)

305-105L 10x50 µg Ask for price

1000bp/1kb DNA ladder (blue, ready-to-use)

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1000bp/1kb DNA ladder (blue, ready-to-use)

305-125 5x50 µg
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100bp plus DNA ladder (blue, ready-to-use)

304-105 50µg/500µl
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100bp plus DNA ladder (blue, ready-to-use)

304-125 5x50 µg
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50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300009 50µg/250µl
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50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300010 5x50 µg
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50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye)

W109-100 - Ask for price

100bp DNA Ladder, 100bp-1.5kb, 11 bands, 100ng/uL, 5uL/load

M7123-100Loads500uL 100Loads (500uL)
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Description: Biotechnology

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M7508-100Loads500uL 100Loads (500uL)
EUR 28
Description: Biotechnology

OneMark 100 DNA Ladder (ready-to-use, 100-3000 bp)

DM101-0100 600 µl
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Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500ul / 500 lanes

PR4100-500 1 PC
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Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500ul / 500 lanes

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Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500ul / 500 lanes

PR4010-500 1 PC
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100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105 50µg/500µl
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100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105L 10x50 µg Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105XL 20x50 µg Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-125 5x50µg
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50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003 50µg/500µl
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50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003L 10x50 µg Ask for price

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003XL 20x50 µg Ask for price

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300004 5x50 µg
EUR 253.2

LMW DNA Ladder, 25bp-700bp, 10 bands, 104ng/uL, 5uL/load

M7101-100Loads500uL 100Loads (500uL)
EUR 44
Description: Biotechnology

ACTGene? DNA Marker, 100 bp Ladder, 11 Fragments, 100 loads

ACT-IDMWD100 each
EUR 178.8

50bp DNA Ladder Plus, 50bp-1kb, 13 bands, 128ng/uL, 5uL/load

M7115-100Loads500uL 100Loads (500uL)
EUR 44
Description: Biotechnology

100 bp DNA Ladder Plus, ready-to-use, 50 ug, 0.5 ug per lane

Z6030001-1 100 lanes
EUR 56

100 bp DNA Ladder Plus, ready-to-use, 250 ug, 0.5 ug per lane

Z6030001-3 500 lanes
EUR 224

100 bp DNA Ladder Plus, loading dye included, 50 ug, 0.5 ug per lane

Z6030001-2 100 lanes
EUR 50

100 bp DNA Ladder Plus, loading dye included, 250 ug, 0.5 ug per lane

Z6030001-4 500 lanes
EUR 198

PRESTAINED PROTEIN LADDER

IB01210 245kDa
EUR 258.78

WSE-7020 EzProtein Ladder

2332346 1unit
EUR 339.6
Description: Pr ot ei n col or ed M ar k er

BriteRuler? Pre-stained Protein Ladder

9306-100 each
EUR 314.4

100bp plus Ladder (no loading dye, 100-1500 bp)

306-005 50µg/250µl
EUR 60

100bp plus Ladder (no loading dye, 100-1500 bp)

306-025 5x50 µg
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Biometric pre-stained protein ladder (5-245 kDa) for Western

BPL11-500 500 ul
EUR 196.8

Un-stained protein ladder (14-116 kDa) for mol wt determination by Western

PMU11-500 50 loads
EUR 110.4

10-240kDa Wide Range 3 Color Prestained Protein Ladder

BG00364 500ul, 500ul
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GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD

310010 1x500µl
EUR 159.6

GENE`s Protein Ladder Extended prestained, 13 bands, 5 - 245kD

310011 5x500µl
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GENE`s Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD

310003 1x500 µl
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GENE`s Protein Ladder PS10 PLUS prestained, 10 bands, 11 - 180kD

310004 5x500µl
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GENE`s Plus Protein Ladder PS11 prestained, 11 bands, 10 - 175kD

310005 1x500µl
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GENE`s Plus Protein Ladder PS11 prestained, 11 bands, 10 - 175kD

310006 5x500µl
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Biometric un-stained protein ladder (10-200 kDa) for mol wt determination by Western

PMU12-50 50 loads
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GENE`s Protein Ladder Wide Range, prestained, 10 bands, 6,5 - 270kD

310020 1x500µl
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GENE`s Protein Ladder Wide Range, prestained, 10 bands, 6,5 - 270kD

310021 5x500µl
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10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained

BZ0010R 500ul, 500ul
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10-250kDa Wide Range Blue-Red-Green Three Color Protein Ladder, Prestained

BZ0011G 500ul, 500ul
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Multicolor, prestained, His-tagged Protein ladder (20 Kda-140 kda) for Western blot

HISM20-C 100 ul
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100 bp LadderDNA Marker HighConcentration

FYD005-500UL 64 µg/ 500 µl Ask for price

Image Green? 1 kb DNA Marker

M1156-500 each
EUR 352.8

SpinStar Total DNA Kit 2.0

BSV-COV-008 100 reactions Ask for price
Description: SpinStar Total DNA Kit 2.0

DNA Polymerase Enzyme (4 kb)

abx071004-10kU 10 kU
EUR 460.8

DNA Polymerase Enzyme (4 kb)

abx071004-3kU 3 kU
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DNA Polymerase Enzyme (4 kb)

abx071004-500U 500 U
EUR 210

DNA Polymerase Enzyme (4 kb)

abx071004-50kU 50 kU
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DNA Polymerase Enzyme (15 kb)

abx071012-250U 250 U
EUR 260.4

DNA Polymerase Enzyme (15 kb)

abx071012-3kU 3 kU
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DNA Polymerase Enzyme (15 kb)

abx071012-500U 500 U
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1.5 kb to 48.5 kb High Range DNA Marker

E255 150ug, 150ug
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DNA Polymerase (6 kb / min) Enzyme

abx071002-3kU 3 kU
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DNA Polymerase (6 kb / min) Enzyme

abx071002-500U 500 U
EUR 226.8

Virus DNA/RNA Extraction Kit 2.0 (Prepackaged)

RM401-01 50 T
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Virus DNA/RNA Extraction Kit 2.0 (Prepackaged)

RM401-02 48 T
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Virus DNA/RNA Extraction Kit 2.0 (Prepackaged)

RM401-04 96 T
EUR 76.8

CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit, 50 preps

CD507 -
EUR 268
Description: Ribonuclease A from bovine pancreas (cow; Bos taurus); Proteinase from Yeast (Pichia pastoris)

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-10kU 10 kU
EUR 610.8

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-3kU 3 kU
EUR 326.4

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-500U 500 U
EUR 226.8

DNA Polymerase (with 2.5 mM dNTPs) (4 kb) Enzyme

abx071003-3kU 3 kU
EUR 410.4

DNA Polymerase (with 2.5 mM dNTPs) (4 kb) Enzyme

abx071003-500U 500 U
EUR 243.6

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-250U 250 U
EUR 276

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-3kU 3 kU
EUR 878.4

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-500U 500 U
EUR 326.4

100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-1 100loading, 100prep
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100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-2 5x100loading, 500prep
EUR 435.84

Leveraging PDA and nested NET-seq reveal widespread genome-wide Pol II pausing at single-nucleotide decision in human cells. Notably, nearly all of Pol II pauses happen outdoors of promoter-proximal gene areas primarily alongside the gene-body of transcribed genes.

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