Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

Anomopoda is the widespread planktonic microcrustacean, which performs a vital function in aquatic ecosystem. There are few research in regards to the evolutionary relationships amongst varied Anomopoda basing on molecular information. Within the current examine, phylogenetic evaluation of eight Anomopoda was carried out. Firstly, the tradition system was developed to breed cladocerans. Through the use of this technique, eight species (Daphnia magna, D. pulex, D. sinensis, Ceriodaphnia reticulata, Moina micrura, Scapholeberis kingi, Simocephalus vetulus and Eurycercus lamellatus) had been purified and cultured stably within the laboratory.

Then, transcriptomic sequences and partial mitochondrial DNA sequences had been each used to reconstruct the phylogenetic tree amongst eight species. Transcriptomic sequences had been sequenced on Illumina Hiseq 2500 platform. After meeting and annotation, transcriptomic sequences had been spliced collectively and aligned for phylogenetic evaluation. Basing on the orthologous genes derived from transcriptomic sequences, the phylogenetic evaluation confirmed that four genera of Daphniidae had been clustered into one group, and among the many four genera, Ceriodaphnia was nearer to Daphnia than Simocephalus, whereas Scapholeberis was farthest from different species. As well as, Eurycercidae was nearer to Daphniidae than Moinidae.

The phylogenetic bushes primarily based on each 12S rRNA and 16S rRNA sequences had been related with that primarily based on transcriptomic sequences. In the meantime, the phylogenetic tree primarily based on 16S rRNA sequences was extra appropriate than that primarily based on 12S rRNA sequences. These outcomes steered that the phylogenetic evaluation basing on the transcriptomic sequences was accessible in cladocerans, which can assist us to successfully perceive the phylogenetic relationships amongst varied cladocerans.

Utility of DNA sequences in anti-counterfeiting: Present progress and challenges

Counterfeiting has by no means been tougher than in the course of the COVID-19 pandemic as counterfeit take a look at kits and therapeutics have been found out there. Present anti-counterfeiting labels have weaknesses: they will both be duplicated simply, are costly or ill-suited for the prevailing advanced provide chains. Whereas RFID tags present for a wonderful different to present anti-counterfeiting strategies, they will show to be costly and different routes involving nanomaterials will be troublesome to encrypt.

A DNA primarily based anticounterfeiting system has vital benefits comparable to relative ease of synthesis and huge information storage skills, together with nice potential in encryption. Though DNA is provided with such helpful properties, main challenges that restrict its real-world anti-counterfeiting functions embody safety in harsh environments, speedy cheap sequence dedication, and its attachment to merchandise. This examine was performed on 62 soil samples collected from livestock stables. The samples had been cultured by way of hair bait approach (HBT).

This assessment elaborates the present progress of DNA primarily based anti-counterfeiting programs and identifies technological gaps that must be stuffed for its sensible utility. Progress made on addressing the first challenges related to the usage of DNA, and potential options are mentioned. Keratinophilic fungi play an vital function within the decomposition of keratinous substances in nature. This capability induces dermatomycosis in each people and livestock. The soil of livestock stables could be a reservoir of keratinophilic fungi. Subsequently, the current examine was performed to isolate and determine keratinophilic fungi within the soil of the livestock stables positioned in Qayen, South Khorasan Province, Iran.

Phylogenetic analysis of eight species of Anomopoda based on transcriptomic and mitochondrial DNA sequences

The impression of flanking sequence options on DNA CpG methylation

Epigenetics and DNA methylation play a pivotal function in lots of processes of the cell and we regularly observe that an aberrant methylation sample characterizes pathologies. On this work we examine the function that the flanking sequences of CGs play within the methylation course of in human. We constructed 4 totally different CG datasets: methylated, unmethylated, and two randomly extracted ones. We evaluated options related to the flanking sequences of these CG units, for various measurement across the CG, via 5 measures accounting for various features of sequence composition complexity and construction.

The evaluation carried out via these measures revealed evident totally different behaviors between methylated and unmethylated probe units. Main variations had been noticed for GC content material and CG dinucleotide frequency in a window measurement of 300-400 bp and for CG self-attraction in 3K bp. It’s exceptional because the impact of methylated CG lasts far more than anticipated removed from the CG.

Full mitochondrial DNA sequence of Norwegian skates ( Raja brachyuran Lafont, 1871) imported to Korea

On this examine, the entire mitochondrial genome of Norwegian skates imported to Korea was sequenced with a round molecule of 17,121 bp, which consisted of 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 switch RNA genes, and a management area (D-loop). And amongst these sequences, 193 bp sequence within the D-loop of the genus Raja steered the opportunity of getting used as a genetic marker for classification of Raja and Dipturus species.

The BI phylogenetic tree by utilizing the nucleotide sequences of 13 PCGs from 15 accessible mitogenomes of household Rajidae confirmed additionally that Norwegian skates imported to Korea kind a bunch with Raja brachyura species with excessive department worth, and that this was a species of Raja brachyura. As above, these outcomes could be anticipated to supply for the additional understanding on the phylogenetic relationship, taxonomic classification and phylogeography of the household Rajidae.

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

50 kb large-Range DNA ladder

308-025 250 µg
EUR 144

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

ACTGene? DNA Marker, 1 kb Ladder, 13 Fragments, 100 loads

ACT-IDMWD1 each
EUR 178.8

ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads

ACT-IDMWD2 each
EUR 178.8

100bp DNA ladder

9K-004-0002 0.5ml
EUR 260.98

OneMark B DNA Ladder (ready-to-use, 250-1000 kb)

DM110-0100 600 µl
EUR 75.6

XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes)

300007 50µg/500µl
EUR 84

XXL DNA ladder ready-to use (2kb - 25 kb, 2 dyes)

300008 5x50 µg
EUR 253.2

100 bp DNA Ladder

MD104-01 250 μl
EUR 132

100 bp DNA Ladder

MD104-02 500 μl
EUR 139.2

iVDye 50bp DNA Ladder

V1001-001 1ml
EUR 218.4

iVDye 50bp DNA Ladder

V1001-025 250ul
EUR 105.6

iVDye 50bp DNA Ladder

V1001-100 4x250ul
EUR 230.4

iVDye 50bp DNA Ladder

V1001-250 10X250ul
EUR 422.4

iVDye 100bp DNA Ladder

V1002-001 1ml
EUR 199.2

iVDye 100bp DNA Ladder

V1002-025 250ul
EUR 96

iVDye 100bp DNA Ladder

V1002-100 4x250ul
EUR 195.6

iVDye 100bp DNA Ladder

V1002-250 10X250ul
EUR 378

iVDye 1Kb DNA Ladder

V1003-001 1ml
EUR 148.8

iVDye 1Kb DNA Ladder

V1003-025 250ul
EUR 91.2

iVDye 1Kb DNA Ladder

V1003-100 4x250ul
EUR 157.2

iVDye 1Kb DNA Ladder

V1003-250 10x250ul
EUR 273.6

iVDye 1Kb DNA Ladder

V1003-500 20x250ul
EUR 457.2

100 bp DNA Ladder

M1158-500 each
EUR 333.6

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300005 86µg/500µl
EUR 84

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006 5x86 µg
EUR 253.2

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006L 10x86 µg
EUR 465.6

One-for-all DNA ladder ready-to-use (100bp - 10 kb, 1 dye)

300006XL 20x86 µg
EUR 894

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Star 1kb DNA Ladder Express

BT10701 500µl
EUR 96
Description: High purity buffer for various PCR applications.

100 bp Ladder DNA Marker

FYD002-500UL 30 µg/ 500 µl Ask for price

50 bp Ladder DNA Marker

FYD004-500UL 32.5 µg/ 500 µl Ask for price

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

Image Green? 1 kb DNA Marker

M1156-500 each
EUR 352.8

Star 100 bp DNA Ladder Express

BA01302 500µl
EUR 96
Description: High purity buffer for various PCR applications.

Enhanced Apoptotic DNA Ladder Detection Kit

K2202-50 50 assays
EUR 568.8

Quick Apoptotic DNA Ladder Detection Kit

K2194-50 50 assays
EUR 568.8

Quick Apoptotic DNA Ladder Detection Kit

K120-50 each
EUR 548.4

Enhanced Apoptotic DNA Ladder Detection Kit

K130-50 each
EUR 529.2

Wide range 100 bp DNA Ladder

M1192-500 each
EUR 333.6

DNA Methylation Antibody Panel Pack I

C20000
  • EUR 458.46
  • EUR 270.60
  • each
  • 2 x 25 ul

100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-1 100loading, 100prep
EUR 143.52

DNA Polymerase Enzyme (4 kb)

abx071004-10kU 10 kU
EUR 460.8

DNA Polymerase Enzyme (4 kb)

abx071004-3kU 3 kU
EUR 276

DNA Polymerase Enzyme (4 kb)

abx071004-500U 500 U
EUR 210

DNA Polymerase Enzyme (4 kb)

abx071004-50kU 50 kU
EUR 1262.4

DNA Polymerase Enzyme (15 kb)

abx071012-250U 250 U
EUR 260.4

DNA Polymerase Enzyme (15 kb)

abx071012-3kU 3 kU
EUR 828

DNA Polymerase Enzyme (15 kb)

abx071012-500U 500 U
EUR 326.4

DNA High Binding Solution

R-1103
  • EUR 267.06
  • EUR 140.80
  • 30 ml
  • 30 ml

100 bp DNA Ladder Plus, ready-to-use, 50 ug, 0.5 ug per lane

Z6030001-1 100 lanes
EUR 130.8

1.5 kb to 48.5 kb High Range DNA Marker

E255 150ug, 150ug
EUR 378.42

100 bp-10 Kb Wide Range DNA Logical Marker, Ready-to-use

M103R-1 100loading, 100prep
EUR 143.52

Renilla Luciferase Assay Kit 2.0

30082-1 150AS
EUR 206.4
Description: Minimum order quantity: 1 unit of 150AS

Firefly Luciferase Assay Kit 2.0

30085-1 150AS
EUR 150
Description: Minimum order quantity: 1 unit of 150AS

20 bp TinyOrange DNA Ladder (105ng/µl)

309-005 500 µl
EUR 129.6

20 bp TinyOrange DNA Ladder (105ng/µl)

309-025 5x500µl
EUR 387.6

1000bp/1kb DNA ladder (no loading dye)

305-005 50µg/250µl
EUR 60

1000bp/1kb DNA ladder (no loading dye)

305-025 5x50µg
EUR 144

1Kb Plus DNA Ladder, Ready-to-use

9K-004-0001 0.5ml
EUR 193.98

Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes

AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes

AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes

AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes

AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes

AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes

AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes

AZ-1155-2 1000 Lanes
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100 bp DNA Ladder 500ul per k

AXY1014 EACH
EUR 145.92

Methylamp 96 DNA Modification Kit 

P-1008
  • EUR 416.70
  • EUR 253.00
  • 96 Samples
  • 96 Samples

DNA Concentrator Kit 

P-1006
  • EUR 303.60
  • EUR 90.20
  • EUR 171.60
  • 100 Samples
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  • 100 Samples

pGreenFire1-NF-kB (plasmid)

TR012PA-1 10 ug
EUR 943.2

pGreenFire1-NF-kB (virus)

TR012VA-1 >2 x 10^6 IFUs
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DNA Polymerase (6 kb / min) Enzyme

abx071002-3kU 3 kU
EUR 360

DNA Polymerase (6 kb / min) Enzyme

abx071002-500U 500 U
EUR 226.8

100bp plus DNA ladder (blue, ready-to-use)

304-105 50µg/500µl
EUR 60

100bp plus DNA ladder (blue, ready-to-use)

304-125 5x50 µg
EUR 144

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105 50µg/500µl
EUR 60

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105L 10x50 µg Ask for price

1000bp/1kb DNA ladder (blue, ready-to-use)

305-105XL 20x50 µg Ask for price

1000bp/1kb DNA ladder (blue, ready-to-use)

305-125 5x50 µg
EUR 144

Methylamp DNA Modification Kit 

P-1001
  • EUR 416.70
  • EUR 149.60
  • EUR 259.60
  • 80 Reactions
  • 40 Reactions
  • 80 Reactions

Supercoiled pHOT-1 DNA

TG2030-1 25 ug
EUR 295.2

Relaxed pHOT-1 DNA

TG2035-1 25 ug
EUR 295.2

MiTeGen Cryo-EM Puck, Generation 2.0 Puck Qty 1

M-CEMG2-1 1 PUCK
EUR 112
Description: MiTeGen Cryo-EM Puck, Generation 2.0 Puck Qty 1FREE Custom puck Barcoding and Engraving (example: ABC-001 to ABC-010) (M-CEMG2-Barcoding)FREE Custom puck color selection - Choose any combination of the following colors: Red, Blue, Purple, Grey, Black, Gold ,Green, Violet, Brown, Orange (M-CEMG2-CustomColor)

Methylamp Universal Methylated DNA Kit 

P-1011
  • EUR 684.66
  • EUR 292.60
  • EUR 442.20
  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

FitAmp Circulating DNA Quantification Kit 

P-1012
  • EUR 595.92
  • EUR 217.80
  • EUR 387.20
  • 96 Assays
  • 48 Assays
  • 96 Assays 

50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300009 50µg/250µl
EUR 60

50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300010 5x50 µg
EUR 144

Anti-NF-kB p65/RELA Antibody

A00284-1 100ug/vial
EUR 400.8

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
  • EUR 149.60
  • EUR 253.00
  • 100 Samples
  • 50 Samples
  • 100 Samples

Methylamp One-Step DNA Modification Kit 

P-1010
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 80 Samples
  • 40 Samples
  • 80 Samples

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Lentivector Plasmid

BLIV511PA-1 10 ug
EUR 963.6

FitAmp General Tissue Section DNA Isolation Kit 

P-1003
  • EUR 754.26
  • EUR 253.00
  • EUR 490.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
  • EUR 702.06
  • EUR 239.80
  • EUR 454.30
  • 100 Samples
  • 50 Samples
  • 100 Samples

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-.1 100 uL/well(10 mM solution)
EUR 4666.8

DNA-Fect

DF37100-1 1 ml
EUR 418.8

DNA-Fect293

DF37293-1 1 ml
EUR 349.2

OneMark 100 DNA Ladder (ready-to-use, 100-3000 bp)

DM101-0100 600 µl
EUR 75.6

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105 50µg/500µl
EUR 60

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105L 10x50 µg Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105XL 20x50 µg Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-125 5x50µg
EUR 144

ACTGene? DNA Marker, 100 bp Ladder, 11 Fragments, 100 loads

ACT-IDMWD100 each
EUR 178.8

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit 

P-3001
  • EUR 1050.06
  • EUR 424.60
  • EUR 712.80
  • 96 Assays
  • 48 Assays
  • 96 Assays

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-10kU 10 kU
EUR 610.8

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-3kU 3 kU
EUR 326.4

DNA Polymerase (with 2.5 mM dNTPs) (1-2 kb / min) Enzyme

abx071005-500U 500 U
EUR 226.8

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Pre-packaged Virus

BLIV511VA-1 >2 x10^6 IFUs
EUR 963.6

NF-kB/Jurkat/GFP Transcriptional Reporter Cell Line

TR850A-1 >2 x 10^6 cells
EUR 4611.6

DNA Polymerase (with 2.5 mM dNTPs) (4 kb) Enzyme

abx071003-3kU 3 kU
EUR 410.4

DNA Polymerase (with 2.5 mM dNTPs) (4 kb) Enzyme

abx071003-500U 500 U
EUR 243.6

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-250U 250 U
EUR 276

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-3kU 3 kU
EUR 878.4

DNA Polymerase (with 2.5 mM dNTPs) (15 kb) Enzyme

abx071013-500U 500 U
EUR 326.4

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid

BLIV713PA-1 10 ug
EUR 963.6

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003 50µg/500µl
EUR 84

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003L 10x50 µg Ask for price

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300003XL 20x50 µg Ask for price

50bp DNA ladder ready-to-use (50bp - 1500 bp, 2 dyes)

300004 5x50 µg
EUR 253.2

Kinetoplast DNA (catenated)

TG2013-1 25 ug
EUR 321.6

Human Genomic DNA 

X11000-1 0.2 ml
EUR 235.4

DNA Cleared Plasma

DCP100A-1 100 ml
EUR 417

NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line

TR860A-1 >2 x 10^6 cells
EUR 3915.6

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus

BLIV713VA-1 >2 x10^6 IFUs
EUR 963.6

KB-5246

HY-19081 5mg
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WSE-7020 EzProtein Ladder

2332346 1unit
EUR 339.6
Description: Pr ot ei n col or ed M ar k er

Anti-DNA-PKCS Antibody

A00645-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DNA-PKCS Antibody (PRKDC) detection.tested for WB in Human, Mouse.

E. coli DNA Gyrase

TG2000G-1 100 units
EUR 388.8

S. aureus DNA Gyrase

TG2000GSA-1 100 units
EUR 483.6

Linear Kinetoplast DNA Marker

TG2018-1 10 ug
EUR 214.8

Decatenated Kinetoplast DNA Marker

TG2020-1 10 ug
EUR 228

Relaxed pRYG DNA Marker

TG2025-1 10 ug
EUR 321.6

Linear pRYG DNA Marker

TG2028-1 10 ug
EUR 214.8

Human TDP2 DNA Substrate

TG2038-1 10 ug
EUR 496.8

Human Brain Genomic DNA  

X11001-1 10 ul
EUR 77

T4 DNA Ligase Buffer

9102-1 each
EUR 105.6

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)

GE620A-1 10 ug
EUR 1443.6

DNA Concentrator Kit 

P-1006-1 50 Samples
EUR 90.2

Oxalic Acid Solution (2.0%)

OQB125 125 ml
EUR 86.4

Oxalic Acid Solution (2.0%)

OQB500 500 ml
EUR 110.4

Oxalic Acid Solution (2.0%)

OQB999 1000 ml
EUR 140.4

TDP1 Human, Tyrosyl-DNA Phosphodiesterase 1 Human Recombinant Protein, Sf9

PROTQ9NUW8-1 Regular: 5ug
EUR 380.4
Description: TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). TDP1 is fused to 9 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques. 

SYBR Safe DNA Gel Stain

A8743-1 1 ml
EUR 193.2

6X DNA Loading Buffer (Blue)

99962-1 4x1.5ML
EUR 124.8
Description: Minimum order quantity: 1 unit of 4x1.5ML

Anti-DNA pol beta Antibody

A01946-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DNA pol beta Antibody (POLB) detection.tested for WB in Human, Mouse, Rat.

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 463.2

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-2 5x100loading, 500prep
EUR 435.84

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)

GE622A-1 10 ug
EUR 1443.6

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP)

GE624A-1 10 ug
EUR 1443.6

BriteRuler? Pre-stained Protein Ladder

9306-100 each
EUR 314.4

KB-R7943 mesylate

A3525-10 10 mg
EUR 172.8
Description: KB-R7943 mesylate is a potent and selective inhibitor of Na+/Ca2+ exchanger (NCE) with IC50 value of 0.7 ?M.

Leveraging PDA and nested NET-seq reveal widespread genome-wide Pol II pausing at single-nucleotide decision in human cells. Notably, nearly all of Pol II pauses happen outdoors of promoter-proximal gene areas primarily alongside the gene-body of transcribed genes.

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