Rat SPON2(Spondin 2) ELISA Kit

High-quality optimized Invitrogen ELISA kits allow you to measure target-specific proteins with confidence, reliability, and consistency. A variety of ELISA kit formats are available which include complete, ready-to-use kits as well as preoptimized reagents to design your own. ELISA kits and antibody pairs are available for a range of different species including human, mouse, rat, nonhuman primate, canine, porcine, bovine, equine, and feline. In addition to the off-the-shelf ELISA formats, special services such as: lot reservation, bulk packaging and custom target development are available for flexibility and convenience as you start your next research project. Search all the Orbigen ELISA kits available with the search bar above.

Cytokine Profiling Antibody Array

Cytokine Profiling Antibody Array features 310 unique antibodies for profiling cytokines, chemokines and related biomarkers in human cells, tissues, serum or culture media. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

KEY FEATURES

  • 310 cytokine related antibodies; 6 replicates per antibody
  • Qualitative/semi-quantitative protein expression profiling of cytokines and related biomarkers
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection

The ELISA based Cytokine Profiling Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Rat RETN / Resistin (CLIA) ELISA Kit – LS-F37648

LS-F37648 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat RETN / Resistin in samples of Plasma and Serum. It is based upon a Sandwich CLIA assay principle and can be used to detect levels of RETN / Resistin as low as 37.5 picograms per milliliter.

Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Rat Spondin 2 (SPON2) ELISA Kit

It is an ELISA Kit for the in vitro quantitative measurement of Rat Spondin 2 (SPON2) concentrations in serum, plasma and other biological fluids. This assay has high sensitivity and excellent specificity for detection of Spondin 2 (SPON2)
No significant cross-reactivity or interference between Spondin 2 (SPON2) and analogues was observed.
Stability
The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Assay Principle

This kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient SPON2 will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow colour is proportional to the SPON2 amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of SPON2 can be calculated.

Kit components

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

Reagent Preparation

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100

Assay Procedure

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 100 µl of diluted standard into the standard wells.
  • 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.
  • 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
  • 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.

Protocol

  1. This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  2. Equilibrate the kit components and samples to room temperature (18 – 25 °C) before use. It is recommended to plot a standard curve for each test.
  3. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
  4.  Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C.
  5.  Remove the cover and discard the liquid.
  6.  Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C.
  7.  Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
  8. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min.
  9.  Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
  10. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
  11. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.

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