The string matching algorithms are among the essential fields in computer science, such as text search, intrusion detection systems, fraud detection, sequence search in bioinformatics. The exact string matching algorithms are divided into two parts: single and multiple. Multiple string matching algorithms involve finding elements of the pattern set P in a given input text T. String matching processes should be done in a time-efficient manner for DNA sequences. As the volume of the text T increases and the number of search patterns increases, the total runtime increases. Efficient algorithms should be selected to perform these search operations as soon as possible. In this study, the Wu-Manber algorithm, one of the multiple exact string matching algorithms, is improved.
Although the Wu-Manber algorithm is effective, it has some limitations, such as hash collisions. In this study, the WM-q algorithm, a version of the Wu-Manber algorithm based on the perfect hash function for DNA sequences, is proposed. String matching is performed using different block lengths provided by the perfect hash function instead of using the fixed block length as in the traditional Wu-Manber algorithm. The proposed approach has been compared with E. Coli and Human Chromosome1 datasets, frequently used in the literature, using multiple exact string matching algorithms. The proposed algorithm gives better results for performance metrics such as the average runtime, the average number of characters and hash comparisons.
2009 saw the first description of Candida auris, a yeast pathogen of humans. C. auris has since grown into a global problem in intensive care settings, where it causes systemic infections in patients with underlying health issues. Recent whole-genome sequencing has discerned five C. auris clades with distinct phenotypic features which display genomic divergence on a DNA sequence and a chromosome structure level. In the absence of sexual reproduction in C. auris, the mechanism(s) behind the rapid genomic evolution of this emerging killer yeast has remained obscure. Yet, one important bit of information about chromosome organization was missing, the identification of the centromeres.
Molecular variation of the cytochrome b DNA and protein sequences in Phytoseiulus macropilis and P. persimilis (Acari: Phytoseiidae) reflect population differentiation
Several phytoseiid mite species are important natural enemies used in biological control strategies. In the present study, Cytb mtDNA sequences of various populations of two species, Phytoseiulus macropolis and P. persimilis, were compared to determine whether the specimens collected in Brazil could belong to P. persimilis as this latter species is reported in South America but not in Brazil. The Cytb marker was used because of its high evolution rate, assumed to capture intraspecific variation. No overlap between intra- and interspecific distances was observed but the distances were quite low for interspecific variation.
This can be due to the particular biology of Phytoseiulus species and this shows the difficulty to apply a universal threshold in genetic distances to conclude about the existence of one or several species. Cytb mtDNA sequences were also considered to assess intraspecific variation. The DNA sequences of P. persimilis populations were very similar, probably because they all originated from the West Palearctic region or because of a prevalence of commercialized specimens in natura. For P. macropilis, higher genetic distances were observed and differentiation was noted according to geographic location and, to a smaller extent, pyrethroid resistance.
To determine how DNA variation might impact the protein function (CytB fragment considered), the amino acid compositions of the populations studied were compared. No diagnostic mutation was observed between pyrethroid resistant and susceptible populations, whereas four mutations were identified between populations of P. macropilis separated by 1300 km (different climatic conditions). The impact of such mutations is discussed but knowledge is scarce, which makes it difficult to root testable hypotheses. The protein analysis clearly opens new perspectives in Phytoseiidae studies.
Characterization of genomic DNA sequence of the candidate gene for FB_Mfu10 associated with fire blight resistance in Malus species
The proposed candidate gene underlying the Malus fusca fire blight resistance locus on chromosome 10 was previously predicted to possess 880 amino acids and 8 exons. Eight base pair (8 bp) insertion/deletion in the first exon potentially distinguished resistant genotypes from susceptible ones. This study aimed at analyzing the candidate gene sequence in another set of original resistant and susceptible progeny, characterizing the sequence in a transgenic line transformed with the candidate gene under its own native promoter, as well as deciphering the potential genomic differences between this candidate gene and its homolog in the ‘Golden Delicious’ doubled haploid genome (GDDH13).
AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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ig SYBR Green qPCR 2X Master Mix - 500 Reactions |
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| 3356 | Intact Genomics | 1/EA | EUR 288 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 138 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 242 |
2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | Ask for price |
Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | Ask for price |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
RT Master Mix for qPCR |
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| HY-K0510 | MedChemExpress | 1 mL (100 rxns) | EUR 291 |
AceQ qPCR Probe Master Mix |
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| Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
AceQ qPCR Probe Master Mix |
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| Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
Entrans 2X qPCR Probe Master Mix |
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| RK21208 | Abclonal | 40 RXN | Ask for price |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-001 | GenDepot | 1ml | EUR 280 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-002 | GenDepot | 2x1ml | EUR 482 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-005 | GenDepot | 5x1ml | EUR 828 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-010 | GenDepot | 10x1ml | EUR 1360 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | EUR 209.5 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | EUR 96.4 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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ABScript II One Step SYBR Green RT-qPCR Kit |
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| RK20404 | Abclonal | 10 RXN | Ask for price |
SYBR Green I |
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| B1747-25 | Biovision | EUR 1164 | |
SYBR Green I |
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| B1747-5 | Biovision | EUR 387 | |
RT Master Mix for qPCR (gDNA digester plus) |
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| HY-K0511 | MedChemExpress | 1 mL (100 rxns) | EUR 315 |
Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | EUR 204 |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | EUR 386 |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | EUR 3116 |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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Fast Plus EvaGreen qPCR Master Mix (200 rxn) |
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| 31020 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Genorise® 2 x qPCR HotStart Master Mix |
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| GR108002 | Genorise Scientific | 2.0 ml | EUR 279 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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| 31041-1 | Biotium | 5x1mL | EUR 272 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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| 31041-20mL | Biotium | 2x10mL | EUR 494 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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| 31041-T | Biotium | 1mL | EUR 114 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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EZSolution? SYBR Green I |
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| B1748-1 | Biovision | EUR 620 | |
EZSolution? SYBR Green I |
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| B1748-500 | Biovision | EUR 430 | |
OPTIONAL GREEN FILTER (572NM) |
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| GDBL-GREEN | CORNING | 1/pk | EUR 1052 |
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Description: Lab Equipment; Axygen Branded EQ |
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Jade? Master Mix |
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| M1105-500 | Biovision | EUR 441 | |
Taqman Master Mix |
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| M1121-500 | Biovision | EUR 441 | |
Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | EUR 123 |
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Description: Minimum order quantity: 1 unit of 1ML |
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Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 119 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 150 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 248 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 272 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 494 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 114 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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amfiSure PCR Master Mix |
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| P0311-010 | GenDepot | 2x50 rxns | EUR 126 |
amfiSure PCR Master Mix |
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| P0311-025 | GenDepot | 5X50 rxns | EUR 165 |
amfiSure PCR Master Mix |
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| P0311-050 | GenDepot | 10X50 rxns | EUR 219 |
amfiSure PCR Master Mix |
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| P0311-100 | GenDepot | 10x1ml | EUR 286 |
amfiSure PCR Master Mix |
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| P0311-125 | GenDepot | 15X50 rxns | EUR 278 |
amfiSure PCR Master Mix |
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| P0311-200 | GenDepot | 20x1ml | EUR 469 |
amfiSure PCR Master Mix |
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| P0311-250 | GenDepot | 50x50 rxns | EUR 752 |
amfiSure PCR Master Mix |
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| P0311-500 | GenDepot | 100x50 rxns | EUR 1258 |
amfiXpand PCR Master Mix |
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| P0331-010 | GenDepot | 2X50 rxns | EUR 151 |
amfiXpand PCR Master Mix |
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| P0331-025 | GenDepot | 5X50 rxns | EUR 273 |
amfiXpand PCR Master Mix |
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| P0331-050 | GenDepot | 10X50 rxns | EUR 448 |
amfiXpand PCR Master Mix |
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| P0331-250 | GenDepot | 50x50 rxns | EUR 1873 |
Accuris Taq Master Mix |
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| PR1001-1000 | Benchmark Scientific | 1 PC | EUR 300.26 |
Accuris Taq Master Mix |
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| PR1001-200 | Benchmark Scientific | 1 PC | EUR 121.62 |
Accuris Taq Master Mix |
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| PR1001-S | Benchmark Scientific | 1 PC | EUR 69.79 |
Taqman Master Mix-iCycler |
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| M1122-500 | Biovision | EUR 441 | |
Taqman Master Mix-ROX |
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| M1124-500 | Biovision | EUR 441 | |
Taqman Master Mix-Multiplex |
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| M1125-500 | Biovision | EUR 441 | |
2 × Taq Master Mix |
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| P111-01 | Vazyme | 5 ml | EUR 117 |
2 × Taq Master Mix |
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| P111-02 | Vazyme | 15 ml | EUR 146 |
2 × Taq Master Mix |
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| P111-03 | Vazyme | 50 ml | EUR 236 |
2 × AceTaq Master Mix |
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| P411-01 | Vazyme | 1 ml | EUR 119 |
2 × AceTaq Master Mix |
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| P411-02 | Vazyme | 5 ml | EUR 158 |
2 × AceTaq Master Mix |
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| P411-03 | Vazyme | 15 ml | EUR 262 |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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| RA910A-1 | SBI | 20 profiles | EUR 1108 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200 | GeneOn | 100 rcs (2,5 ml) | EUR 159 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200L | GeneOn | 500 rcs (12,5 ml) | EUR 612 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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HotTaq Probe qPCR Mix (ROX) |
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| BT11001 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq Probe qPCR Mix (Capillary) |
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| BT11003 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq Probe qPCR Universal Mix |
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| BT11004 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (ROX) |
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| BT11101 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (Capillary) |
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| BT11103 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Universal Mix |
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| BT11104 | Bioatlas | 250rxn | EUR 138 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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amfiSure Prime PCR Master Mix |
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| P1311-025 | GenDepot | 5X50 rxns | EUR 161 |
amfiSure Prime PCR Master Mix |
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| P1311-050 | GenDepot | 10X50 rxns | EUR 219 |
amfiSure Prime PCR Master Mix |
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| P1311-125 | GenDepot | 15X50 rxns | EUR 273 |
amfiSure Prime PCR Master Mix |
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| P1311-500 | GenDepot | 100x50 rxns | EUR 1238 |
amfiSure Advanced PCR Master Mix |
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| P2311-025 | GenDepot | 5X50 rxns | EUR 165 |
amfiSure Advanced PCR Master Mix |
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| P2311-050 | GenDepot | 10X50 rxns | EUR 223 |
amfiSure Advanced PCR Master Mix |
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| P2311-125 | GenDepot | 15X50 rxns | EUR 282 |
amfiSure Advanced PCR Master Mix |
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| P2311-250 | GenDepot | 50x50 rxns | EUR 752 |
amfiSure Advanced PCR Master Mix |
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| P2311-500 | GenDepot | 100x50 rxns | EUR 1279 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-050 | GenDepot | 50 rxns | EUR 415 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-100 | GenDepot | 2X50 rxns | EUR 847 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-200 | GenDepot | 4X50 rxns | EUR 1211 |
Seamless cloning Master Mix (Kit) |
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| B632219 | Bio Basic | 40RXN, 40prep | EUR 266.63 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-1000 | Benchmark Scientific | 1 PC | EUR 827.41 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-200 | Benchmark Scientific | 1 PC | EUR 242.84 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-S | Benchmark Scientific | 1 PC | EUR 69.79 |
Taqman Master Mix-Low ROX |
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| M1123-500 | Biovision | EUR 441 | |
2 × Taq Plus Master Mix |
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| P211-01 | Vazyme | 5 ml | EUR 134 |
2 × Taq Plus Master Mix |
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| P211-02 | Vazyme | 15 ml | EUR 193 |
2 × Taq Plus Master Mix |
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| P211-03 | Vazyme | 50 ml | EUR 389 |
2 × Rapid Taq Master Mix |
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| P222-01 | Vazyme | 5 ml (5×1ml) | EUR 120 |
2 × Rapid Taq Master Mix |
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| P222-02 | Vazyme | 15 ml (15×1ml) | EUR 153 |
2 × Rapid Taq Master Mix |
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| P222-03 | Vazyme | 50 ml (50 x 1 ml) | EUR 257 |
2 × Rapid Taq Master Mix |
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| P222-04 | Vazyme | 50 ml (10×5ml) | EUR 257 |
2 × Vazyme LAmp Master Mix |
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| P311-01 | Vazyme | 1 ml | EUR 137 |
2 × Vazyme LAmp Master Mix |
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| P311-02 | Vazyme | 5 ml | EUR 212 |
2 × Vazyme LAmp Master Mix |
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| P311-03 | Vazyme | 15 ml | EUR 401 |
2 × Phanta Max Master Mix |
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| P515-01 | Vazyme | 1 ml | EUR 145 |
2 × Phanta Max Master Mix |
|||
| P515-02 | Vazyme | 5 ml | EUR 269 |
2 × Phanta Max Master Mix |
|||
| P515-03 | Vazyme | 15 ml | EUR 575 |
AceQ U+ Probe Master Mix |
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| Q113-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 267 |
AceQ U+ Probe Master Mix |
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| Q113-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 842 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160 | GeneOn | 100 rcs (2,5 ml) | EUR 159 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160L | GeneOn | 500 rcs (12,5 ml) | EUR 612 |
SYBR Green I Nucleic Acid Gel Stain |
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| HY-K1004 | MedChemExpress | 100 μL | EUR 113 |
Eztime Real - time PCRPremix 2x,SYBR Green |
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| FYT103-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
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| FYT103-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
SensiFAST SYBR Lo-ROX Mix, 50 mL |
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| BIO-94050 | Bioline | 5000 rxn | Ask for price |
SensiFAST SYBR No-ROX Mix, 50 mL |
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| BIO-98050 | Bioline | 5000 rxn | Ask for price |
Top Green qPCR SuperMix (+Dye II) |
|||
| 20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
|||
| 20-abx098891 | Abbexa |
|
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HotTaq Probe qPCR Mix (no ROX) |
|||
| BT11002 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
|||
HotTaq EvaGreen qPCR Mix (no ROX) |
|||
| BT11102 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
|||
ExoELISA-ULTRA Complete Kit (CD63 detection) |
|||
| EXEL-ULTRA-CD63-1 | SBI | 96 reactions | EUR 713 |
ExoELISA-ULTRA Complete Kit (CD81 detection) |
|||
| EXEL-ULTRA-CD81-1 | SBI | 96 reactions | EUR 713 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
|||
| S210 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
Maximo Dry-Master Mix (Lyophilized Polymerase) |
|||
| S295 | GeneOn | 192 rcs (20µl, in plates or tubes) | EUR 133 |
amfiSure ONE PCR Master Mix(2X) |
|||
| P7000-005 | GenDepot | 5x1 ml | EUR 219 |
amfiSure ONE PCR Master Mix(2X) |
|||
| P7000-010 | GenDepot | 10x1 ml | EUR 317 |
amfiSure ONE PCR Master Mix(2X) |
|||
| P7000-050 | GenDepot | 50x1 ml | EUR 1211 |
amfiSure ONE PCR Master Mix(2X) |
|||
| P7000-100 | GenDepot | 100x1 ml | EUR 2102 |
amfiRivert II cDNA Synthesis Master Mix |
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| R5500-050 | GenDepot | 50 rxns | EUR 374 |
amfiRivert II cDNA Synthesis Master Mix |
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| R5500-100 | GenDepot | 2x50rxns | EUR 577 |
amfiRivert cDNA Synthesis Prime Master Mix |
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| R5610-050 | GenDepot | 50 rxns | EUR 412 |
amfiRivert cDNA Synthesis Prime Master Mix |
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| R5610-100 | GenDepot | 2x50 rxns | EUR 671 |
amfiRivert cDNA Synthesis Prime Master Mix |
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| R5610-200 | GenDepot | 4X50 rxns | EUR 1211 |
amfiRivert cDNA Synthesis Prime Master Mix |
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| R5610-500 | GenDepot | 10x50 rxns | EUR 2548 |
2xEs Taq Master Mix (with Dyes) |
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| W0690-1 | 101Bio | - | Ask for price |
2xEs Taq Master Mix (with Dyes) |
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| W0690-5 | 101Bio | - | Ask for price |
Sequences of amplicons of part of the candidate gene amplified in two progenies that showed resistant and susceptible fire blight phenotypes, confirmed the 8 bp insertion that distinguishes susceptible and resistant progenies. The transgenic line was positive for the candidate gene sequence, confirming a successful transfer into the background of apple cultivar ‘Pinova’, and possessed the same genomic sequence as the progeny with a resistant phenotype. Sequence analysis showed that the homolog gene on GDDH13 possesses a significant 18 bp deletion in exon 1 leading to a difference of 15 amino acid from the protein sequence of the candidate gene.