The string matching algorithms are among the essential fields in computer science, such as text search, intrusion detection systems, fraud detection, sequence search in bioinformatics. The exact string matching algorithms are divided into two parts: single and multiple. Multiple string matching algorithms involve finding elements of the pattern set P in a given input text T. String matching processes should be done in a time-efficient manner for DNA sequences. As the volume of the text T increases and the number of search patterns increases, the total runtime increases. Efficient algorithms should be selected to perform these search operations as soon as possible. In this study, the Wu-Manber algorithm, one of the multiple exact string matching algorithms, is improved.
Although the Wu-Manber algorithm is effective, it has some limitations, such as hash collisions. In this study, the WM-q algorithm, a version of the Wu-Manber algorithm based on the perfect hash function for DNA sequences, is proposed. String matching is performed using different block lengths provided by the perfect hash function instead of using the fixed block length as in the traditional Wu-Manber algorithm. The proposed approach has been compared with E. Coli and Human Chromosome1 datasets, frequently used in the literature, using multiple exact string matching algorithms. The proposed algorithm gives better results for performance metrics such as the average runtime, the average number of characters and hash comparisons.
2009 saw the first description of Candida auris, a yeast pathogen of humans. C. auris has since grown into a global problem in intensive care settings, where it causes systemic infections in patients with underlying health issues. Recent whole-genome sequencing has discerned five C. auris clades with distinct phenotypic features which display genomic divergence on a DNA sequence and a chromosome structure level. In the absence of sexual reproduction in C. auris, the mechanism(s) behind the rapid genomic evolution of this emerging killer yeast has remained obscure. Yet, one important bit of information about chromosome organization was missing, the identification of the centromeres.
Molecular variation of the cytochrome b DNA and protein sequences in Phytoseiulus macropilis and P. persimilis (Acari: Phytoseiidae) reflect population differentiation
Several phytoseiid mite species are important natural enemies used in biological control strategies. In the present study, Cytb mtDNA sequences of various populations of two species, Phytoseiulus macropolis and P. persimilis, were compared to determine whether the specimens collected in Brazil could belong to P. persimilis as this latter species is reported in South America but not in Brazil. The Cytb marker was used because of its high evolution rate, assumed to capture intraspecific variation. No overlap between intra- and interspecific distances was observed but the distances were quite low for interspecific variation.
This can be due to the particular biology of Phytoseiulus species and this shows the difficulty to apply a universal threshold in genetic distances to conclude about the existence of one or several species. Cytb mtDNA sequences were also considered to assess intraspecific variation. The DNA sequences of P. persimilis populations were very similar, probably because they all originated from the West Palearctic region or because of a prevalence of commercialized specimens in natura. For P. macropilis, higher genetic distances were observed and differentiation was noted according to geographic location and, to a smaller extent, pyrethroid resistance.
To determine how DNA variation might impact the protein function (CytB fragment considered), the amino acid compositions of the populations studied were compared. No diagnostic mutation was observed between pyrethroid resistant and susceptible populations, whereas four mutations were identified between populations of P. macropilis separated by 1300 km (different climatic conditions). The impact of such mutations is discussed but knowledge is scarce, which makes it difficult to root testable hypotheses. The protein analysis clearly opens new perspectives in Phytoseiidae studies.
Characterization of genomic DNA sequence of the candidate gene for FB_Mfu10 associated with fire blight resistance in Malus species
The proposed candidate gene underlying the Malus fusca fire blight resistance locus on chromosome 10 was previously predicted to possess 880 amino acids and 8 exons. Eight base pair (8 bp) insertion/deletion in the first exon potentially distinguished resistant genotypes from susceptible ones. This study aimed at analyzing the candidate gene sequence in another set of original resistant and susceptible progeny, characterizing the sequence in a transgenic line transformed with the candidate gene under its own native promoter, as well as deciphering the potential genomic differences between this candidate gene and its homolog in the ‘Golden Delicious’ doubled haploid genome (GDDH13).
AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 290.4 |
2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | Ask for price |
Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | Ask for price |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
RT Master Mix for qPCR |
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| HY-K0510 | MedChemExpress | 1 mL (100 rxns) | EUR 349.2 |
AceQ qPCR Probe Master Mix |
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| Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR Probe Master Mix |
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| Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
Entrans 2X qPCR Probe Master Mix |
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| RK21208 | Abclonal | 40 RXN | Ask for price |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17270 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17271 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17272 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17273 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17274 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17275 | AAT Bioquest | 5 mL | EUR 308 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-001 | GenDepot | 1ml | EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-002 | GenDepot | 2x1ml | EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-005 | GenDepot | 5x1ml | EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-010 | GenDepot | 10x1ml | EUR 1632 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 789.36 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 197.34 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | EUR 308 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | EUR 251.4 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | EUR 115.68 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
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PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
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SYBR Green I |
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| B1747-25 | Biovision | each | EUR 1396.8 |
SYBR Green I |
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| B1747-5 | Biovision | each | EUR 464.4 |
Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | EUR 244.8 |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | EUR 463.2 |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | EUR 3739.2 |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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RT Master Mix for qPCR (gDNA digester plus) |
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| HY-K0511 | MedChemExpress | 1 mL (100 rxns) | EUR 378 |
Genorise® 2 x qPCR HotStart Master Mix |
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| GR108002 | Genorise Scientific | 2.0 ml | EUR 334.8 |
ABScript II One Step SYBR Green RT-qPCR Kit |
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| RK20404 | Abclonal | 10 RXN | Ask for price |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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| 31041-1 | Biotium | 5x1mL | EUR 326.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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| 31041-20mL | Biotium | 2x10mL | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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| 31041-T | Biotium | 1mL | EUR 136.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Jade? Master Mix |
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| M1105-500 | Biovision | each | EUR 529.2 |
Taqman Master Mix |
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| M1121-500 | Biovision | each | EUR 529.2 |
EZSolution? SYBR Green I |
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| B1748-1 | Biovision | each | EUR 744 |
EZSolution? SYBR Green I |
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| B1748-500 | Biovision | each | EUR 516 |
OPTIONAL GREEN FILTER (572NM) |
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| GDBL-GREEN | CORNING | 1/pk | EUR 1262.4 |
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Description: Lab Equipment; Axygen Branded EQ |
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Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | EUR 147.6 |
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Description: Minimum order quantity: 1 unit of 1ML |
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Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
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| CRISPY100A-1 | SBI | 200 rxn | EUR 412 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17285 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17287 | AAT Bioquest | 5 mL | EUR 308 |
Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 142.8 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 180 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 297.6 |
CytoTrace™ Ultra Green |
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| 21800 | AAT Bioquest | 1 mg | EUR 278 |
amfiSure PCR Master Mix |
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| P0311-010 | GenDepot | 2x50 rxns | EUR 151.2 |
amfiSure PCR Master Mix |
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| P0311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure PCR Master Mix |
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| P0311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure PCR Master Mix |
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| P0311-100 | GenDepot | 10x1ml | EUR 343.2 |
amfiSure PCR Master Mix |
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| P0311-125 | GenDepot | 15X50 rxns | EUR 333.6 |
amfiSure PCR Master Mix |
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| P0311-200 | GenDepot | 20x1ml | EUR 562.8 |
amfiSure PCR Master Mix |
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| P0311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure PCR Master Mix |
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| P0311-500 | GenDepot | 100x50 rxns | EUR 1509.6 |
amfiXpand PCR Master Mix |
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| P0331-010 | GenDepot | 2X50 rxns | EUR 181.2 |
amfiXpand PCR Master Mix |
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| P0331-025 | GenDepot | 5X50 rxns | EUR 327.6 |
amfiXpand PCR Master Mix |
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| P0331-050 | GenDepot | 10X50 rxns | EUR 537.6 |
amfiXpand PCR Master Mix |
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| P0331-250 | GenDepot | 50x50 rxns | EUR 2247.6 |
2 × Taq Master Mix |
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| P111-01 | Vazyme | 5 ml | EUR 140.4 |
2 × Taq Master Mix |
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| P111-02 | Vazyme | 15 ml | EUR 175.2 |
2 × Taq Master Mix |
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| P111-03 | Vazyme | 50 ml | EUR 283.2 |
2 × AceTaq Master Mix |
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| P411-01 | Vazyme | 1 ml | EUR 142.8 |
2 × AceTaq Master Mix |
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| P411-02 | Vazyme | 5 ml | EUR 189.6 |
2 × AceTaq Master Mix |
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| P411-03 | Vazyme | 15 ml | EUR 314.4 |
Accuris Taq Master Mix |
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| PR1001-1000 | Benchmark Scientific | 1 PC | EUR 360.31 |
Accuris Taq Master Mix |
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| PR1001-200 | Benchmark Scientific | 1 PC | EUR 145.94 |
Accuris Taq Master Mix |
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| PR1001-S | Benchmark Scientific | 1 PC | EUR 83.75 |
Taqman Master Mix-iCycler |
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| M1122-500 | Biovision | each | EUR 529.2 |
Taqman Master Mix-ROX |
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| M1124-500 | Biovision | each | EUR 529.2 |
Taqman Master Mix-Multiplex |
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| M1125-500 | Biovision | each | EUR 529.2 |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 326.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 136.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17288 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17289 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17290 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17291 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17292 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293 | AAT Bioquest | 5 mL | EUR 308 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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Volatile Organics Mix |
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| 60-BIG-MIX | Scientific Laboratory Supplies | 1ML | EUR 120.84 |
5 Component Mix |
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| AOAC-MIX-1 | Scientific Laboratory Supplies | EACH | EUR 117.42 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374L | MiTeGen | 10 x 1,25 ml | EUR 720.29 |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374S | MiTeGen | 2 x 1,25 ml | EUR 180.14 |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375L | MiTeGen | 10 x 1,25 ml | EUR 864.5 |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375S | MiTeGen | 2 x 1,25 ml | EUR 216.19 |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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Seamless cloning Master Mix (Kit) |
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| B632219 | Bio Basic | 40RXN, 40prep | EUR 319.96 |
amfiSure Prime PCR Master Mix |
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| P1311-025 | GenDepot | 5X50 rxns | EUR 193.2 |
amfiSure Prime PCR Master Mix |
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| P1311-050 | GenDepot | 10X50 rxns | EUR 262.8 |
amfiSure Prime PCR Master Mix |
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| P1311-125 | GenDepot | 15X50 rxns | EUR 327.6 |
amfiSure Prime PCR Master Mix |
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| P1311-500 | GenDepot | 100x50 rxns | EUR 1485.6 |
2 × Taq Plus Master Mix |
|||
| P211-01 | Vazyme | 5 ml | EUR 160.8 |
2 × Taq Plus Master Mix |
|||
| P211-02 | Vazyme | 15 ml | EUR 231.6 |
2 × Taq Plus Master Mix |
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| P211-03 | Vazyme | 50 ml | EUR 466.8 |
2 × Rapid Taq Master Mix |
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| P222-01 | Vazyme | 5 ml (5×1ml) | EUR 144 |
2 × Rapid Taq Master Mix |
|||
| P222-02 | Vazyme | 15 ml (15×1ml) | EUR 183.6 |
2 × Rapid Taq Master Mix |
|||
| P222-03 | Vazyme | 50 ml (50 x 1 ml) | EUR 308.4 |
2 × Rapid Taq Master Mix |
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| P222-04 | Vazyme | 50 ml (10×5ml) | EUR 308.4 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-025 | GenDepot | 5X50 rxns | EUR 198 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-050 | GenDepot | 10X50 rxns | EUR 267.6 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-125 | GenDepot | 15X50 rxns | EUR 338.4 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-250 | GenDepot | 50x50 rxns | EUR 902.4 |
amfiSure Advanced PCR Master Mix |
|||
| P2311-500 | GenDepot | 100x50 rxns | EUR 1534.8 |
2 × Vazyme LAmp Master Mix |
|||
| P311-01 | Vazyme | 1 ml | EUR 164.4 |
2 × Vazyme LAmp Master Mix |
|||
| P311-02 | Vazyme | 5 ml | EUR 254.4 |
2 × Vazyme LAmp Master Mix |
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| P311-03 | Vazyme | 15 ml | EUR 481.2 |
2 × Phanta Max Master Mix |
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| P515-01 | Vazyme | 1 ml | EUR 174 |
2 × Phanta Max Master Mix |
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| P515-02 | Vazyme | 5 ml | EUR 322.8 |
2 × Phanta Max Master Mix |
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| P515-03 | Vazyme | 15 ml | EUR 690 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-1000 | Benchmark Scientific | 1 PC | EUR 992.89 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-200 | Benchmark Scientific | 1 PC | EUR 291.41 |
Accuris Taq Plus Master Mix |
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| PR1001-TP-S | Benchmark Scientific | 1 PC | EUR 83.75 |
AceQ U+ Probe Master Mix |
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| Q113-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 320.4 |
AceQ U+ Probe Master Mix |
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| Q113-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 1010.4 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-050 | GenDepot | 50 rxns | EUR 498 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-100 | GenDepot | 2X50 rxns | EUR 1016.4 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-200 | GenDepot | 4X50 rxns | EUR 1453.2 |
Taqman Master Mix-Low ROX |
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| M1123-500 | Biovision | each | EUR 529.2 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200 | GeneOn | 100 rcs (2,5 ml) | EUR 190.8 |
Sequences of amplicons of part of the candidate gene amplified in two progenies that showed resistant and susceptible fire blight phenotypes, confirmed the 8 bp insertion that distinguishes susceptible and resistant progenies. The transgenic line was positive for the candidate gene sequence, confirming a successful transfer into the background of apple cultivar ‘Pinova’, and possessed the same genomic sequence as the progeny with a resistant phenotype. Sequence analysis showed that the homolog gene on GDDH13 possesses a significant 18 bp deletion in exon 1 leading to a difference of 15 amino acid from the protein sequence of the candidate gene.