The string matching algorithms are among the essential fields in computer science, such as text search, intrusion detection systems, fraud detection, sequence search in bioinformatics. The exact string matching algorithms are divided into two parts: single and multiple. Multiple string matching algorithms involve finding elements of the pattern set P in a given input text T. String matching processes should be done in a time-efficient manner for DNA sequences. As the volume of the text T increases and the number of search patterns increases, the total runtime increases. Efficient algorithms should be selected to perform these search operations as soon as possible. In this study, the Wu-Manber algorithm, one of the multiple exact string matching algorithms, is improved.
Although the Wu-Manber algorithm is effective, it has some limitations, such as hash collisions. In this study, the WM-q algorithm, a version of the Wu-Manber algorithm based on the perfect hash function for DNA sequences, is proposed. String matching is performed using different block lengths provided by the perfect hash function instead of using the fixed block length as in the traditional Wu-Manber algorithm. The proposed approach has been compared with E. Coli and Human Chromosome1 datasets, frequently used in the literature, using multiple exact string matching algorithms. The proposed algorithm gives better results for performance metrics such as the average runtime, the average number of characters and hash comparisons.
2009 saw the first description of Candida auris, a yeast pathogen of humans. C. auris has since grown into a global problem in intensive care settings, where it causes systemic infections in patients with underlying health issues. Recent whole-genome sequencing has discerned five C. auris clades with distinct phenotypic features which display genomic divergence on a DNA sequence and a chromosome structure level. In the absence of sexual reproduction in C. auris, the mechanism(s) behind the rapid genomic evolution of this emerging killer yeast has remained obscure. Yet, one important bit of information about chromosome organization was missing, the identification of the centromeres.
Molecular variation of the cytochrome b DNA and protein sequences in Phytoseiulus macropilis and P. persimilis (Acari: Phytoseiidae) reflect population differentiation
Several phytoseiid mite species are important natural enemies used in biological control strategies. In the present study, Cytb mtDNA sequences of various populations of two species, Phytoseiulus macropolis and P. persimilis, were compared to determine whether the specimens collected in Brazil could belong to P. persimilis as this latter species is reported in South America but not in Brazil. The Cytb marker was used because of its high evolution rate, assumed to capture intraspecific variation. No overlap between intra- and interspecific distances was observed but the distances were quite low for interspecific variation.
This can be due to the particular biology of Phytoseiulus species and this shows the difficulty to apply a universal threshold in genetic distances to conclude about the existence of one or several species. Cytb mtDNA sequences were also considered to assess intraspecific variation. The DNA sequences of P. persimilis populations were very similar, probably because they all originated from the West Palearctic region or because of a prevalence of commercialized specimens in natura. For P. macropilis, higher genetic distances were observed and differentiation was noted according to geographic location and, to a smaller extent, pyrethroid resistance.
To determine how DNA variation might impact the protein function (CytB fragment considered), the amino acid compositions of the populations studied were compared. No diagnostic mutation was observed between pyrethroid resistant and susceptible populations, whereas four mutations were identified between populations of P. macropilis separated by 1300 km (different climatic conditions). The impact of such mutations is discussed but knowledge is scarce, which makes it difficult to root testable hypotheses. The protein analysis clearly opens new perspectives in Phytoseiidae studies.
Characterization of genomic DNA sequence of the candidate gene for FB_Mfu10 associated with fire blight resistance in Malus species
The proposed candidate gene underlying the Malus fusca fire blight resistance locus on chromosome 10 was previously predicted to possess 880 amino acids and 8 exons. Eight base pair (8 bp) insertion/deletion in the first exon potentially distinguished resistant genotypes from susceptible ones. This study aimed at analyzing the candidate gene sequence in another set of original resistant and susceptible progeny, characterizing the sequence in a transgenic line transformed with the candidate gene under its own native promoter, as well as deciphering the potential genomic differences between this candidate gene and its homolog in the ‘Golden Delicious’ doubled haploid genome (GDDH13).
SYBR Green qPCR Master Mix (No ROX) |
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| C0006-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| C0006-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| C0006-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
SYBR Green qPCR Master Mix (High ROX) |
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| C0007-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 135.6 |
2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
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| THK0221 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
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| THK0222 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
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| THK0223 | Westburg | each | EUR 3324.5 |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
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| THK0241 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
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| THK0242 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
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| THK0243 | Westburg | each | Ask for price |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
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| THK0251 | Westburg | each | EUR 232.17 |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
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| THK0252 | Westburg | each | EUR 1046.4 |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
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| THK0253 | Westburg | each | Ask for price |
BioEasy Master Mix (SYBR Green) |
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| TRI-B25L1 | TRI Biotech | 200T | EUR 403.2 |
SYBR Green cRNA PCR Master Mix |
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| SGM002 | Signosis | 2 ml | EUR 174 |
EnoGene Green qPCR Master Mix |
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| E31M001 | EnoGene | 500 reaction | EUR 75 |
ChamQ SYBR qPCR Master Mix |
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| Q311-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR qPCR Master Mix |
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| Q311-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ SYBR qPCR Master Mix |
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| Q311-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
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| Q311-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix |
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| Q311-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
Apex qPCR GREEN Master Mix |
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| 42-116PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 341.44 |
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Description: 2X, Without ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-117PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 2926.74 |
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Description: 2X, Without ROX, 50ml |
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Apex qPCR GREEN Master Mix |
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| 42-118PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 338.88 |
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Description: 2X, Low ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-119PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 2926.74 |
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Description: 2X, Low ROX, 50ml |
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Apex qPCR GREEN Master Mix |
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| 42-120PG | Genesee Scientific | 400 x 25µl Reactions/Unit | EUR 341.44 |
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Description: 2X, High ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-121PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | EUR 3095.57 |
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Description: 2X, High ROX, 50ml |
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BioEasy Master Mix (SYBR Green, No ROX) |
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| TRI-B25L1D | TRI Biotech | 200T | EUR 403.2 |
BioEasy Master Mix (SYBR Green, low ROX) |
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| TRI-B25L1C | TRI Biotech | 200T | EUR 403.2 |
BioEasy Master Mix (SYBR Green, high ROX) |
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| TRI-B25L1B | TRI Biotech | 200T | EUR 403.2 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02-500rxns | Vazyme | 500 rxns | EUR 158.14 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 688.5 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03-2500rxns | Vazyme | 2,500 rxns | EUR 712.94 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02-500rxns | Vazyme | 500 rxns | EUR 158.14 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 688.5 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03-2500rxns | Vazyme | 2,500 rxns | EUR 712.94 |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-01-125rxns | Vazyme | 125 rxns | EUR 40.28 |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 145 |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-02-500rxns | Vazyme | 500 rxns | EUR 150.15 |
HotStart™ 2X Green qPCR Master Mix |
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| K1070-25 | ApexBio | 25ml | EUR 392 |
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Description: Reverse Transcription & PCR|qPCR |
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HotStart™ 2X Green qPCR Master Mix |
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| K1070-5 | ApexBio | 5ml | EUR 124 |
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Description: Reverse Transcription & PCR|qPCR |
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HotStart™ 2X Green qPCR Master Mix |
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| K1070-50 | ApexBio | 50ml | EUR 768 |
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Description: Reverse Transcription & PCR|qPCR |
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2×SYBR Green qPCR Mix |
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| E37F021-10x5mL | EnoGene | 10×5mL | EUR 1360 |
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Description: DsDNA, ssDNA, RNA quantitative detection kit |
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2×SYBR Green qPCR Mix |
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| E37F021-5mL | EnoGene | 5mL | EUR 227 |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix |
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| MBS8579840-200Tests | MyBiosource | 200Tests | EUR 285 |
2×SYBR Green qPCR Mix |
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| MBS8579840-50Tests | MyBiosource | 50Tests | EUR 180 |
2×SYBR Green qPCR Mix |
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| MBS8579840-5x200Tests | MyBiosource | 5x200Tests | EUR 1230 |
2×SYBR Green qPCR Mix |
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| MBS8579840-5x50Tests | MyBiosource | 5x50Tests | EUR 770 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 161 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02-500rxns | Vazyme | 500 rxns | EUR 166.72 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 725 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03-2500rxns | Vazyme | 2,500 rxns | EUR 750.74 |
miRNA SYBR Green qPCR Mix |
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| 4308A | Unibiotest | 100 Tests*20μL | EUR 80 |
miRNA SYBR Green qPCR Mix |
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| 4308B | Unibiotest | 200 Tests*20μL | EUR 150 |
miRNA SYBR Green qPCR Mix |
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| 4308C | Unibiotest | 500 Tests*20μL | EUR 300 |
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
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| QLMM10 | Vivantis | each | Ask for price |
HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-25 | ApexBio | 25ml | EUR 424 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-5 | ApexBio | 5ml | EUR 132 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-50 | ApexBio | 50ml | EUR 826.4 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-25 | ApexBio | 25ml | EUR 392 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-5 | ApexBio | 5ml | EUR 124 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-50 | ApexBio | 50ml | EUR 752 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-25 | ApexBio | 25ml | EUR 424 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-5 | ApexBio | 5ml | EUR 132 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-50 | ApexBio | 50ml | EUR 826.4 |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
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| QLMM12 | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
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| QLMM17 | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
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| QLMM02 | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns |
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| QLMM07 | Vivantis | each | Ask for price |
Hieff® miRNA Universal qPCR SYBR Master Mix |
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| 11171ES03 | Yeasen Biotechnology | 1 mL | EUR 35 |
Hieff® miRNA Universal qPCR SYBR Master Mix |
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| 11171ES08 | Yeasen Biotechnology | 5 mL | EUR 150 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17270-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
ABsolute SYBR Green QPCR Mix 800 rxns |
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| AB-1158/B | Westburg | each | Ask for price |
ABsolute SYBR Green QPCR Mix 200 rxns |
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| AB-1159 | Westburg | each | EUR 529.74 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272 | AAT Bioquest | 1 mL | EUR 203 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17273 | AAT Bioquest | 5 mL | EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17273-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17274 | AAT Bioquest | 1 mL | EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
|||
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|||
| 17274-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275 | AAT Bioquest | 5 mL | EUR 508 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2×SYBR Green qPCR Mix(including ROX) |
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| E37F022-10x5mL | EnoGene | 10×5mL | EUR 1360 |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix(including ROX) |
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| E37F022-5mL | EnoGene | 5mL | EUR 227 |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-200Tests | MyBiosource | 200Tests | EUR 285 |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-50Tests | MyBiosource | 50Tests | EUR 180 |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-5x200Tests | MyBiosource | 5x200Tests | EUR 1230 |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-5x50Tests | MyBiosource | 5x50Tests | EUR 770 |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | EUR 203 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | EUR 508 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES03 | Yeasen Biotechnology | 1 mL | EUR 55 |
Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES08 | Yeasen Biotechnology | 5×1mL | EUR 245 |
Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES25 | Yeasen Biotechnology | 5×5mL | EUR 1040 |
Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | EUR 76.3 |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | EUR 203 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | EUR 508 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | EUR 203 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | EUR 508 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2X Universal SYBR Green Fast qPCR Mix |
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| MBS9141672-25mL | MyBiosource | 25mL | EUR 755 |
2X Universal SYBR Green Fast qPCR Mix |
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| MBS9141672-5mL | MyBiosource | 5mL | EUR 255 |
2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | EUR 21.26 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302A | Unibiotest | 1 mL | EUR 80 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302B | Unibiotest | 5*1 mL | EUR 250 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302C | Unibiotest | 50*1 mL | EUR 1400 |
Sequences of amplicons of part of the candidate gene amplified in two progenies that showed resistant and susceptible fire blight phenotypes, confirmed the 8 bp insertion that distinguishes susceptible and resistant progenies. The transgenic line was positive for the candidate gene sequence, confirming a successful transfer into the background of apple cultivar ‘Pinova’, and possessed the same genomic sequence as the progeny with a resistant phenotype. Sequence analysis showed that the homolog gene on GDDH13 possesses a significant 18 bp deletion in exon 1 leading to a difference of 15 amino acid from the protein sequence of the candidate gene.