The WM-q multiple exact string matching algorithm for DNA sequences

The WM-q multiple exact string matching algorithm for DNA sequences

The string matching algorithms are among the essential fields in computer science, such as text search, intrusion detection systems, fraud detection, sequence search in bioinformatics. The exact string matching algorithms are divided into two parts: single and multiple. Multiple string matching algorithms involve finding elements of the pattern set P in a given input text T. String matching processes should be done in a time-efficient manner for DNA sequences. As the volume of the text T increases and the number of search patterns increases, the total runtime increases. Efficient algorithms should be selected to perform these search operations as soon as possible. In this study, the Wu-Manber algorithm, one of the multiple exact string matching algorithms, is improved.

Although the Wu-Manber algorithm is effective, it has some limitations, such as hash collisions. In this study, the WM-q algorithm, a version of the Wu-Manber algorithm based on the perfect hash function for DNA sequences, is proposed. String matching is performed using different block lengths provided by the perfect hash function instead of using the fixed block length as in the traditional Wu-Manber algorithm. The proposed approach has been compared with E. Coli and Human Chromosome1 datasets, frequently used in the literature, using multiple exact string matching algorithms. The proposed algorithm gives better results for performance metrics such as the average runtime, the average number of characters and hash comparisons.

2009 saw the first description of Candida auris, a yeast pathogen of humans. C. auris has since grown into a global problem in intensive care settings, where it causes systemic infections in patients with underlying health issues. Recent whole-genome sequencing has discerned five C. auris clades with distinct phenotypic features which display genomic divergence on a DNA sequence and a chromosome structure level. In the absence of sexual reproduction in C. auris, the mechanism(s) behind the rapid genomic evolution of this emerging killer yeast has remained obscure. Yet, one important bit of information about chromosome organization was missing, the identification of the centromeres.

Molecular variation of the cytochrome b DNA and protein sequences in Phytoseiulus macropilis and P. persimilis (Acari: Phytoseiidae) reflect population differentiation

Several phytoseiid mite species are important natural enemies used in biological control strategies. In the present study, Cytb mtDNA sequences of various populations of two species, Phytoseiulus macropolis and P. persimilis, were compared to determine whether the specimens collected in Brazil could belong to P. persimilis as this latter species is reported in South America but not in Brazil. The Cytb marker was used because of its high evolution rate, assumed to capture intraspecific variation. No overlap between intra- and interspecific distances was observed but the distances were quite low for interspecific variation.

This can be due to the particular biology of Phytoseiulus species and this shows the difficulty to apply a universal threshold in genetic distances to conclude about the existence of one or several species. Cytb mtDNA sequences were also considered to assess intraspecific variation. The DNA sequences of P. persimilis populations were very similar, probably because they all originated from the West Palearctic region or because of a prevalence of commercialized specimens in natura. For P. macropilis, higher genetic distances were observed and differentiation was noted according to geographic location and, to a smaller extent, pyrethroid resistance.

The WM-q multiple exact string matching algorithm for DNA sequences

To determine how DNA variation might impact the protein function (CytB fragment considered), the amino acid compositions of the populations studied were compared. No diagnostic mutation was observed between pyrethroid resistant and susceptible populations, whereas four mutations were identified between populations of P. macropilis separated by 1300 km (different climatic conditions). The impact of such mutations is discussed but knowledge is scarce, which makes it difficult to root testable hypotheses. The protein analysis clearly opens new perspectives in Phytoseiidae studies.

Characterization of genomic DNA sequence of the candidate gene for FB_Mfu10 associated with fire blight resistance in Malus species

 The proposed candidate gene underlying the Malus fusca fire blight resistance locus on chromosome 10 was previously predicted to possess 880 amino acids and 8 exons. Eight base pair (8 bp) insertion/deletion in the first exon potentially distinguished resistant genotypes from susceptible ones. This study aimed at analyzing the candidate gene sequence in another set of original resistant and susceptible progeny, characterizing the sequence in a transgenic line transformed with the candidate gene under its own native promoter, as well as deciphering the potential genomic differences between this candidate gene and its homolog in the ‘Golden Delicious’ doubled haploid genome (GDDH13).

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-25 25ml
EUR 420

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-5 5ml
EUR 120

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-50 50ml
EUR 840

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-25 25ml
EUR 420
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-5 5ml
EUR 120
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-50 50ml
EUR 840
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 507.2

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 775.2

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 265.2

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 775.2

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 434.8

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 128.8

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 580

miRNA Universal SYBR® qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 165.6

miRNA Universal SYBR® qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 290.4

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 507.2

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 507.2

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 507.2

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL Ask for price

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL Ask for price

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17270 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17271 5 mL
EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17272 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17273 5 mL
EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17274 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17275 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17276 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17277 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17278 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17279 5 mL
EUR 308

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17280 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17281 5 mL
EUR 308

BrightCycle Universal SYBR Green qPCR Mix with UDG

RK21219 5mL
EUR 475

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)

RK21205 1 ml Ask for price

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 1ml
EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 2x1ml
EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 5x1ml
EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 10x1ml
EUR 1632

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 349.2

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 775.2

1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield)

W141 200 rxn
EUR 199

HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
EUR 251.4

HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 115.68

1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility)

W142 200 rxn
EUR 199

Entrans 2X qPCR Probe Master Mix

RK21208 40 RXN Ask for price

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)

31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)

31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

EZGreen 2x qPCR Master Mix, 40Rxns-50ul

A4157-1ML40Rxns 1ML (40Rxns)
EUR 55
Description: Biotechnology

Green-2-Go qPCR Mastermix (500X20ul Rxn)

QPCR004-S 4X1.25ml
EUR 303.25

ABScript III RT Master Mix for qPCR

RK20428 10RXN
EUR 82.5

ABScript Neo RT Master Mix for qPCR

RK20432 100RXN
EUR 17.5

Entrans 2X qPCR Probe Master Mix V2

RK21212 50RXN
EUR 67.5

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)

QPCR001-R 4X1.25ml
EUR 303.25

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)

31041-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn)

QPCR003-iC 4X1.25ml
EUR 303.25

RT Master Mix for qPCR (gDNA digester plus)

HY-K0511 1 mL (100 rxns)
EUR 378

Entrans 2X qPCR Probe Master Mix with UDG

RK21222 50RXN
EUR 71

Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn)

QPCR006-NODYE 4X1.25ml
EUR 326.22

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)

31041-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)

31041-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Genorise® 2 x qPCR HotStart Master Mix

GR108002 2.0 ml
EUR 279

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)

QPCR002-L 4X1.25ml
EUR 303.25

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17282-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17283-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17284-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17285-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

One Step SYBR Green RT-PCR Mix

EQ007 20 µL x 100 rxn
EUR 248

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*

17286-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*

17287-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17282 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17283 5 mL
EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17284 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17285 5 mL
EUR 308

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)

31020-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17286 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17287 5 mL
EUR 308

Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn)

QPCR005-NODYE 4X1.25ml
EUR 303.25

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul

A4183-4x125ML500Rxns 4x1.25ML (500Rxns)
EUR 200.2
Description: Biotechnology

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)

31014-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

31014-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)

31015-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)

31015-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289 5 mL
EUR 308

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190L 500 rcs (12,5 ml)
EUR 627.6

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293 5 mL
EUR 308

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)

31003-T 1ML
EUR 147.6
Description: Minimum order quantity: 1 unit of 1ML

ABScript III RT Master Mix for qPCR with gDNA Remover

RK20429 10RXN
EUR 20

ABScript Neo RT Master Mix for qPCR with gDNA Remover

RK20433 100RXN
EUR 30

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)

31014-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)

31015-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials

S200 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials

S200L 500 rcs (12,5 ml)
EUR 734.4

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)

9-31045
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)

31042-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)

31042-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)

31042-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)

9-31046
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)

31014 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150L 500 rcs (12,5 ml)
EUR 627.6

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-372L 10 x 1,25 ml
EUR 818
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-372S 2 x 1,25 ml
EUR 205
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation

CRISPY100A-1 200 rxn
EUR 412

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials

S160 100 rcs (2,5 ml)
EUR 190.8

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials

S160L 500 rcs (12,5 ml)
EUR 734.4

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)

31015 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-373L 10 x 1,25 ml
EUR 818
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-373S 2 x 1,25 ml
EUR 205
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-375L 10 x 1,25 ml
EUR 982
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-375S 2 x 1,25 ml
EUR 246
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials

S210 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials

S210L 500 rcs (12,5 ml)
EUR 627.6

2×SYBR-Green-qPCR-Mix

E37F021 10×5mL
EUR 1360
Description: DsDNA,-ssDNA,-RNA-quantitative-detection-kit

SYBR Green I

B1747-25 each
EUR 1396.8

SYBR Green I

B1747-5 each
EUR 464.4

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-374L 10 x 1,25 ml
EUR 818
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-374S 2 x 1,25 ml
EUR 205
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials

S175 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials

S175L 500 rcs (12,5 ml)
EUR 627.6

Sequences of amplicons of part of the candidate gene amplified in two progenies that showed resistant and susceptible fire blight phenotypes, confirmed the 8 bp insertion that distinguishes susceptible and resistant progenies. The transgenic line was positive for the candidate gene sequence, confirming a successful transfer into the background of apple cultivar ‘Pinova’, and possessed the same genomic sequence as the progeny with a resistant phenotype. Sequence analysis showed that the homolog gene on GDDH13 possesses a significant 18 bp deletion in exon 1 leading to a difference of 15 amino acid from the protein sequence of the candidate gene.

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