We report two cholesterol-modified oligonucleotides to be used as inner controls for on-DNA reactions in the course of the pooled levels of a DNA-encoded chemical library (DECL) synthesis. As these cholesterol-tagged oligonucleotides are chromatographically separable from regular DECL intermediates, they are often immediately monitored by mass spectrometry to trace response development inside a fancy pool […]
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Mismatch-introduced DNA probes constructed on the basis of thermodynamic analysis enable the discrimination of single nucleotide variants
Genotyping of single nucleotide variants (SNVs) has enabled the evaluation of disease-related threat elements and considerably improved the efficiency of prognosis and prognosis. In the meantime, genotyping of SNVs is difficult as a result of excessive sequence similarity between wild-type (WT) and SNV. To extend the discrimination between WT and SNV, probes are modified with […]
H-NS and ToxT inversely control cholera toxin production by binding to overlapping DNA sequences
Vibrio cholerae infects human hosts following ingestion of contaminated meals or water, ensuing within the extreme diarrheal illness cholera. The watery diarrhea that’s attribute of the illness is immediately brought on by manufacturing of cholera toxin (CT). A fancy regulatory cascade controls manufacturing of CT and different virulence elements. Nevertheless, finally a single protein, ToxT, immediately […]